Institute of Life Science 1 internal Atrium view up

Dr Eylan Yutuc

Darlithydd – Rheolwr Rhaglenni MSc, Biomedical Sciences

eylan.yutuc@abertawe.ac.uk

017
Llawr Gwaelod
Y Sefydliad Gwyddor Bywyd 2
Campws Singleton

Ar gael ar gyfer Goruchwyliaeth Ôl-raddedig

Dolenni Ymchwil

https://orcid.org/0000-0001-9971-1950

ResearchGate

Google Scholar

Scopus

Trosolwg

Astudiodd Eylan ar gyfer ei radd BSc mewn Gwyddor Fiofeddygol ym Mhrifysgol Brunel Llundain a graddiodd yn 2011. Yn ystod ei gyfnod israddedig, roedd e'n rhan o waith syntheseiddio proteinau ailgyfuno a phuro'r protein cyflenwi C1q mewn hilion bacterol yng Nghanolfan Heintiau, Imiwnedd a Mecanweithiau Clefydau, Prifysgol Brunel. Yn y pen draw, gwnaeth ei brosiect blwyddyn olaf yn yr un labordy a archwiliodd ryngweithiadau'r protein C-adweitheddol yn y cyfnod acíwt dynol gyda chlwyf Aspergilws dan oruchwyliaeth Dr Uday Kishore.

Dechreuodd Eylan ei PhD mewn Astudiaethau Meddygol a Gofal Iechyd gyda grŵp Griffiths-Wang Abertawe ym mis Ionawr 2013 lle gwnaeth ganolbwyntio ar nodi, meintioli a dadansoddi metabolion colesterol wedi'u hocsideiddio a grëir gan lymffosytau CD4 + T wedi'u meithrin in vitro gan ddefnyddio technegau megis echdynnu lipidau, tarddu â chymorth ensymau, echdynnu cyfnod solet a chromatograffeg hylif perfformiad uchel - sbectrometreg màs.

Gan ehangu ei sgiliau fel Ymchwilydd Ôl-ddoethurol, mae ef bellach wedi symud ymlaen i ddatblygu a chymhwyso technegau sbectrometreg màs ac offer niwrobioleg, gan ystyried perthynas lipidau sterolau mewn niwroffisioleg a niwropatholeg. Mae Eylan yn aelod o Gymdeithas Sbectrometreg Màs Prydain a’r Gymdeithas Gemeg Frenhinol.

Meysydd Arbenigedd

· Biocemeg Lipidau
· Sbectrometreg Màs Cromatograffeg Hylif
· MALDI MS
· Niwrowyddoniaeth
· Imiwnoleg

Uchafbwyntiau Gyrfa

Ymchwil

Ei brosiect presennol, gyda'r Athro William J. Griffiths a'r Athro Yuqin Wang, yw adeiladu atlas sterol tri dimensiwn o ymennydd y llygoden er mwyn cyd-fynd â gwaith cynyddu gwybodaeth am effeithiau metaboledd sterol mewn niwrobioleg.

Cydweithrediadau

Cyhoeddiadau

Uchafbwyntiau Ymchwil

Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Skibinski, D., Howell, O., Wang, Y., & Griffiths, W. (2020). Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism. Proceedings of the National Academy of Sciences, 117(11), 5749-5760.
https://doi.org/10.1073/pnas.1917421117, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53792
Zang, R., Case, J., Yutuc, E., Ma, X., Shen, S., Castro, M., Liu, Z., Zeng, Q., Zhao, H., Son, J., Rothlauf, P., Kreutzberger, A., Hou, G., Zhang, H., Bose, S., Wang, X., Vahey, M., Mani, K., Griffiths, W...., & Ding, S. (2020). Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. Proceedings of the National Academy of Sciences, 117(50), 32105-32113.
https://doi.org/10.1073/pnas.2012197117, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55583
Angelini, R., Yutuc, E., Wyatt, M., Newton, J., YUSUF, F., Griffiths, L., Cooze, B., Assad, D., Frache, G., Rao, W., Allen, L., Korade, Z., Nguyen, T., Rathnayake, R., Cologna, S., Howell, O., Clench, M., Wang, Y., & Griffiths, W. (2021). Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging. Analytical Chemistry, 93(11), 4932-4943.
https://doi.org/10.1021/acs.analchem.0c05399, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56326
http://dx.doi.org/10.1021/acs.analchem.0c05399
Yutuc, E., Dickson, A., Pacciarini, M., Griffiths, L., Baker, P., Connell, L., Öhman, A., Forsgren, L., Trupp, M., Vilarinho, S., Khalil, Y., Clayton, P., Sari, S., Dalgic, B., Höflinger, P., Schöls, L., Griffiths, W., & Wang, Y. (2021). Deep mining of oxysterols and cholestenoic acids in human plasma and cerebrospinal fluid: Quantification using isotope dilution mass spectrometry. Analytica Chimica Acta, 1154, 338259
https://doi.org/10.1016/j.aca.2021.338259, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56163
Griffiths, W., Abdel-Khalik, J., Moore, S., Wijeyekoon, R., Crick, P., Yutuc, E., Farrell, K., Breen, D., Williams-Gray, C., Theofilopoulos, S., Arenas, E., Trupp, M., Barker, R., & Wang, Y. (2021). The Cerebrospinal Fluid Profile of Cholesterol Metabolites in Parkinson’s Disease and Their Association With Disease State and Clinical Features. Frontiers in Aging Neuroscience, 13, 685594
https://doi.org/10.3389/fnagi.2021.685594, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57043
Wang, Y., Yutuc, E., & Griffiths, W. (2021). Neuro‐oxysterols and neuro‐sterols as ligands to nuclear receptors, GPCRs, ligand‐gated ion channels and other protein receptors. British Journal of Pharmacology, 178(16), 3176-3193.
https://doi.org/10.1111/bph.15191, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54644
Wang, Y., Yutuc, E., & Griffiths, W. (2021). Cholesterol metabolism pathways – are the intermediates more important than the products?. The FEBS Journal, 288(12), 3727-3745.
https://doi.org/10.1111/febs.15727, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56158
Höflinger, P., Hauser, S., Yutuc, E., Hengel, H., Griffiths, L., Radelfahr, F., Howell, O., Wang, Y., Connor, S., Duell, P., DeBarber, A., Martus, P., Lütjohann, D., Griffiths, W., & Schöls, L. (2021). Metabolic profiling in serum, cerebrospinal fluid, and brain of patients with cerebrotendinous xanthomatosis. Journal of Lipid Research, 62, 100078
https://doi.org/10.1016/j.jlr.2021.100078, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56747
Abdel-Khalik, J., Hearn, T., Dickson, A., Crick, P., Yutuc, E., Austin-Muttitt, K., Bigger, B., Morris, A., Shackleton, C., Clayton, P., Iida, T., Sircar, R., Rohatgi, R., Marschall, H., Sjövall, J., Björkhem, I., Mullins, J., Griffiths, W., & Wang, Y. (2021). Bile acid biosynthesis in Smith-Lemli-Opitz syndrome bypassing cholesterol: Potential importance of pathway intermediates. The Journal of Steroid Biochemistry and Molecular Biology, 206, 105794
https://doi.org/10.1016/j.jsbmb.2020.105794, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55690
Wang, Y., Yutuc, E., & Griffiths, W. (2020). Standardizing and increasing the utility of lipidomics: a look to the next decade. Expert Review of Proteomics, 17(10), 699-717.
https://doi.org/10.1080/14789450.2020.1847086, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55597
Hudson, N., Reverter, A., Griffiths, W., Yutuc, E., Wang, Y., Jeanes, A., McWilliam, S., Pethick, D., & Greenwood, P. (2020). Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle. BMC Genomics, 21(1)
https://doi.org/10.1186/s12864-020-6505-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53490
Griffiths, W., Yutuc, E., Davies, D., Dickson, A., Angelini, R., Assad, D., Frache, G., & Wang, Y. (2020). CHAPTER 1. Lipidomics Basics. New Developments in Mass Spectrometry (pp. 1-24). Royal Society of Chemistry
https://doi.org/10.1039/9781788013109-00001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53525
Yutuc, E., Griffiths, W., Yutuc, E., Abdel-Khalik, J., Crick, P., Hearn, T., Dickson, A., Bigger, B., Wu, T., Goenka, A., Ghosh, A., Jones, S., Covey, D., Ory, D., & Wang, Y. (2019). Metabolism of Non-Enzymatically Derived Oxysterols: Clues from sterol metabolic disorders. Free Radical Biology and Medicine
https://doi.org/10.1016/j.freeradbiomed.2019.04.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50061
Yutuc, E., Crick, P., Yutuc, E., Abdel-Khalik, J., Saeed, A., Betsholtz, C., Genove, G., Björkhem, I., Wang, Y., & Griffiths, W. (2019). Formation and metabolism of oxysterols and cholestenoic acids found in the mouse circulation: Lessons learnt from deuterium-enrichment experiments and the CYP46A1 transgenic mouse. The Journal of Steroid Biochemistry and Molecular Biology, 195, 105475
https://doi.org/10.1016/j.jsbmb.2019.105475, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51994
Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Skibinski, D., Howell, O., Wang, Y., & Griffiths, W. (2019). Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism
https://doi.org/10.1101/450973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53208
Griffiths, W., Abdel-Khalik, J., Yutuc, E., Roman, G., Warner, M., Gustafsson, J., & Wang, Y. (2019). Concentrations of bile acid precursors in cerebrospinal fluid of Alzheimer's disease patients. Free Radical Biology and Medicine, 134, 42-52.
https://doi.org/10.1016/j.freeradbiomed.2018.12.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48010
Meljon, A., Crick, P., Yutuc, E., Yau, J., Seckl, J., Theofilopoulos, S., Arenas, E., Wang, Y., & Griffiths, W. (2019). Mining for Oxysterols in Cyp7b1−/− Mouse Brain and Plasma: Relevance to Spastic Paraplegia Type 5. Biomolecules, 9(4), 149
https://doi.org/10.3390/biom9040149, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50029
Yutuc, E., Abdel-Khalik, J., Crick, P., Yutuc, E., Wang, Y., & Griffiths, W. (2019). Developing an Enzyme-Assisted Derivatization Method for Analysis of C27 Bile Alcohols and Acids by Electrospray Ionization-Mass Spectrometry. Molecules, 24(3), 597
https://doi.org/10.3390/molecules24030597, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48910
Theofilopoulos, S., Oliveira, W., Yang, S., Yutuc, E., Saeed, A., Abdel-Khalik, J., Ullgren, A., Cedazo-Minguez, A., Björkhem, I., Wang, Y., Griffiths, W., & Arenas, E. (2019). 24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. Journal of Biological Chemistry, 294(11), 4169-4176.
https://doi.org/10.1074/jbc.ra118.005639, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48277
http://dx.doi.org/10.1074/jbc.ra118.005639
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Abdel-Khalik, J., Crick, P., Yutuc, E., DeBarber, A., Duell, P., Steiner, R., Laina, I., Wang, Y., & Griffiths, W. (2018). Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma. Biochimie
https://doi.org/10.1016/j.biochi.2018.06.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa40916
Griffiths, W., Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Howell, O., & Wang, Y. (2018). Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation-Robotic Liquid Micro-Extraction Surface Analysis-Liquid Chromatography Mass Spectrometry
https://doi.org/10.1101/450973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48402
Griffiths, W., Abdel-Khalik, J., Crick, P., Yutuc, E., Ogundare, M., Bigger, B., Morris, A., Shackleton, C., Clayton, P., Iida, T., Sircar, R., Marschall, H., Sjoval, J., Bjorkhem, I., Rohatgi, R., & Wang, Y. (2018). Bile Acid Biosynthesis Avoiding Cholesterol
https://doi.org/10.1101/385419, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48403
Griffiths, W., Gilmore, I., Yutuc, E., Abdel-Khalik, J., Crick, P., Hearn, T., Dickson, A., Bigger, B., Wu, T., Goenka, A., Ghosh, A., Jones, S., & Wang, Y. (2018). Identification of unusual oxysterols and bile acids with 7-oxo or 3β,5α,6β-trihydroxy functions in human plasma by charge-tagging mass spectrometry with multistage fragmentation. Journal of Lipid Research, 59(6), 1058-1070.
https://doi.org/10.1194/jlr.d083246, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa39367
Griffiths, W., Hearn, T., Crick, P., Abdel-Khalik, J., Dickson, A., Yutuc, E., & Wang, Y. (2017). Charge-tagging liquid chromatography–mass spectrometry methodology targeting oxysterol diastereoisomers. Chemistry and Physics of Lipids, 207, 69-80.
https://doi.org/10.1016/j.chemphyslip.2017.04.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32978
Griffiths, W., Abdel-Khalik, J., Yutuc, E., Morgan, A., Gilmore, I., Hearn, T., & Wang, Y. (2017). Cholesterolomics: An update. Analytical Biochemistry
https://doi.org/10.1016/j.ab.2017.01.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31729
Abdel-Khalik, J., Yutuc, E., Crick, P., Gustafsson, J., Warner, M., Roman, G., Talbot, K., Gray, E., Griffiths, W., Turner, M., & Wang, Y. (2017). Defective cholesterol metabolism in amyotrophic lateral sclerosis. Journal of Lipid Research, jlr.P071639
https://doi.org/10.1194/jlr.P071639, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa30987
Robertson, K., Hsieh, W., Forster, T., Blanc, M., Lu, H., Crick, P., Yutuc, E., Watterson, S., Martin, K., Griffiths, S., Enright, A., Yamamoto, M., Pradeepa, M., Lennox, K., Behlke, M., Talbot, S., Haas, J., Dölken, L., Griffiths, W...., & Ghazal, P. (2016). An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. PLOS Biology, 14(3), e1002364
https://doi.org/10.1371/journal.pbio.1002364, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa27043
Griffiths, W., Abdel-Khalik, J., Crick, P., Yutuc, E., & Wang, Y. (2016). New methods for analysis of oxysterols and related compounds by LC–MS. The Journal of Steroid Biochemistry and Molecular Biology, 162, 4-26.
https://doi.org/10.1016/j.jsbmb.2015.11.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25192
Griffiths, W., Abdel-Khalik, J., Hearn, T., Yutuc, E., Morgan, A., Wang, Y., Griffiths, W., Wang, Y., Morgan, A., & Yutuc, E. (2016). Current trends in oxysterol research. Biochemical Society Transactions, 44(2), 652-658.
https://doi.org/10.1042/BST20150255, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa27117
Angelini, R., Yutuc, E., Wyatt, M., Newton, J., Yusuf, F., Griffiths, L., Cooze, B., Assad, D., Frache, G., Rao, W., Allen, L., Korade, Z., Nguyen, T., Rathnayake, R., Cologna, S., Howell, O., Clench, M., Wang, Y., & Griffiths, W. (2020). Visualising Cholesterol in Brain by On-Tissue Derivatisation and Quantitative Mass Spectrometry Imaging. bioRxiv
https://doi.org/10.1101/2020.11.06.369447, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60125
Hennegan, J., Bryant, A., Griffiths, L., Trigano, M., Bartley, O., Bartlett, J., Minahan, C., Oliveira, W., Yutuc, E., Ntikas, S., Bartsocas, C., Markouri, M., Antoniadou, E., Laina, I., Howell, O., Li, M., Wang, Y., Griffiths, W., Lane, E...., & Theofilopoulos, S. (2024). Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development. iScience, 27(1), 108670
https://doi.org/10.1016/j.isci.2023.108670, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65068
Griffiths, W., Yutuc, E., & Wang, Y. (2023). Mass Spectrometry Imaging of Cholesterol and Oxysterols. Implication of Oxysterols and Phytosterols in Aging and Human Diseases (pp. 73-87). Springer International Publishing
https://doi.org/10.1007/978-3-031-43883-7_5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64898
Griffiths, W., Yutuc, E., Ali Asgari, M., & Wang, Y. (2023). Role of Bile Acid Pathway Intermediates in Pathology of CTX
https://doi.org/10.5281/zenodo.10050199, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64910
Dickson, A., Yutuc, E., Thornton, C., Dunford, J., Oppermann, U., Wang, Y., & Griffiths, W. (2023). HSD3B1 is an oxysterol 3β-hydroxysteroid dehydrogenase in human placenta. Open Biology, 13(5)
https://doi.org/10.1098/rsob.220313, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61629
http://dx.doi.org/10.1098/rsob.220313
Dickson, A., Yutuc, E., Thornton, C., Wang, Y., & Griffiths, W. (2022). Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry. Frontiers in Endocrinology, 13
https://doi.org/10.3389/fendo.2022.1031013, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61630
Haider, A., Zhao, C., Wang, L., Xiao, Z., Rong, J., Xia, X., Chen, Z., Pfister, S., Mast, N., Yutuc, E., Chen, J., Li, Y., Shao, T., Warnock, G., Dawoud, A., Connors, T., Oakley, D., Wei, H., Wang, J...., & Liang, S. (2022). Assessment of cholesterol homeostasis in the living human brain. Science Translational Medicine, 14(665)
https://doi.org/10.1126/scitranslmed.adc9967, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61471
Yutuc, E. (2017). Analysis of Sterols in Human T Helper Cells
https://doi.org/10.23889/SUthesis.60641, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60641

Holl Ymchwil

Erthyglau Cylchgronau

Hennegan, J., Bryant, A., Griffiths, L., Trigano, M., Bartley, O., Bartlett, J., Minahan, C., Oliveira, W., Yutuc, E., Ntikas, S., Bartsocas, C., Markouri, M., Antoniadou, E., Laina, I., Howell, O., Li, M., Wang, Y., Griffiths, W., Lane, E...., & Theofilopoulos, S. (2024). Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development. iScience, 27(1), 108670
https://doi.org/10.1016/j.isci.2023.108670, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65068
Dickson, A., Yutuc, E., Thornton, C., Dunford, J., Oppermann, U., Wang, Y., & Griffiths, W. (2023). HSD3B1 is an oxysterol 3β-hydroxysteroid dehydrogenase in human placenta. Open Biology, 13(5)
https://doi.org/10.1098/rsob.220313, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61629
http://dx.doi.org/10.1098/rsob.220313
Dickson, A., Yutuc, E., Thornton, C., Wang, Y., & Griffiths, W. (2022). Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry. Frontiers in Endocrinology, 13
https://doi.org/10.3389/fendo.2022.1031013, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61630
Haider, A., Zhao, C., Wang, L., Xiao, Z., Rong, J., Xia, X., Chen, Z., Pfister, S., Mast, N., Yutuc, E., Chen, J., Li, Y., Shao, T., Warnock, G., Dawoud, A., Connors, T., Oakley, D., Wei, H., Wang, J...., & Liang, S. (2022). Assessment of cholesterol homeostasis in the living human brain. Science Translational Medicine, 14(665)
https://doi.org/10.1126/scitranslmed.adc9967, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61471
Griffiths, W., Abdel-Khalik, J., Moore, S., Wijeyekoon, R., Crick, P., Yutuc, E., Farrell, K., Breen, D., Williams-Gray, C., Theofilopoulos, S., Arenas, E., Trupp, M., Barker, R., & Wang, Y. (2021). The Cerebrospinal Fluid Profile of Cholesterol Metabolites in Parkinson’s Disease and Their Association With Disease State and Clinical Features. Frontiers in Aging Neuroscience, 13, 685594
https://doi.org/10.3389/fnagi.2021.685594, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57043
Wang, Y., Yutuc, E., & Griffiths, W. (2021). Neuro‐oxysterols and neuro‐sterols as ligands to nuclear receptors, GPCRs, ligand‐gated ion channels and other protein receptors. British Journal of Pharmacology, 178(16), 3176-3193.
https://doi.org/10.1111/bph.15191, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54644
Wang, Y., Yutuc, E., & Griffiths, W. (2021). Cholesterol metabolism pathways – are the intermediates more important than the products?. The FEBS Journal, 288(12), 3727-3745.
https://doi.org/10.1111/febs.15727, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56158
Höflinger, P., Hauser, S., Yutuc, E., Hengel, H., Griffiths, L., Radelfahr, F., Howell, O., Wang, Y., Connor, S., Duell, P., DeBarber, A., Martus, P., Lütjohann, D., Griffiths, W., & Schöls, L. (2021). Metabolic profiling in serum, cerebrospinal fluid, and brain of patients with cerebrotendinous xanthomatosis. Journal of Lipid Research, 62, 100078
https://doi.org/10.1016/j.jlr.2021.100078, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56747
Yutuc, E., Dickson, A., Pacciarini, M., Griffiths, L., Baker, P., Connell, L., Öhman, A., Forsgren, L., Trupp, M., Vilarinho, S., Khalil, Y., Clayton, P., Sari, S., Dalgic, B., Höflinger, P., Schöls, L., Griffiths, W., & Wang, Y. (2021). Deep mining of oxysterols and cholestenoic acids in human plasma and cerebrospinal fluid: Quantification using isotope dilution mass spectrometry. Analytica Chimica Acta, 1154, 338259
https://doi.org/10.1016/j.aca.2021.338259, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56163
Angelini, R., Yutuc, E., Wyatt, M., Newton, J., YUSUF, F., Griffiths, L., Cooze, B., Assad, D., Frache, G., Rao, W., Allen, L., Korade, Z., Nguyen, T., Rathnayake, R., Cologna, S., Howell, O., Clench, M., Wang, Y., & Griffiths, W. (2021). Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging. Analytical Chemistry, 93(11), 4932-4943.
https://doi.org/10.1021/acs.analchem.0c05399, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56326
http://dx.doi.org/10.1021/acs.analchem.0c05399
Abdel-Khalik, J., Hearn, T., Dickson, A., Crick, P., Yutuc, E., Austin-Muttitt, K., Bigger, B., Morris, A., Shackleton, C., Clayton, P., Iida, T., Sircar, R., Rohatgi, R., Marschall, H., Sjövall, J., Björkhem, I., Mullins, J., Griffiths, W., & Wang, Y. (2021). Bile acid biosynthesis in Smith-Lemli-Opitz syndrome bypassing cholesterol: Potential importance of pathway intermediates. The Journal of Steroid Biochemistry and Molecular Biology, 206, 105794
https://doi.org/10.1016/j.jsbmb.2020.105794, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55690
Zang, R., Case, J., Yutuc, E., Ma, X., Shen, S., Castro, M., Liu, Z., Zeng, Q., Zhao, H., Son, J., Rothlauf, P., Kreutzberger, A., Hou, G., Zhang, H., Bose, S., Wang, X., Vahey, M., Mani, K., Griffiths, W...., & Ding, S. (2020). Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. Proceedings of the National Academy of Sciences, 117(50), 32105-32113.
https://doi.org/10.1073/pnas.2012197117, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55583
Wang, Y., Yutuc, E., & Griffiths, W. (2020). Standardizing and increasing the utility of lipidomics: a look to the next decade. Expert Review of Proteomics, 17(10), 699-717.
https://doi.org/10.1080/14789450.2020.1847086, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55597
Hudson, N., Reverter, A., Griffiths, W., Yutuc, E., Wang, Y., Jeanes, A., McWilliam, S., Pethick, D., & Greenwood, P. (2020). Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle. BMC Genomics, 21(1)
https://doi.org/10.1186/s12864-020-6505-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53490
Angelini, R., Yutuc, E., Wyatt, M., Newton, J., Yusuf, F., Griffiths, L., Cooze, B., Assad, D., Frache, G., Rao, W., Allen, L., Korade, Z., Nguyen, T., Rathnayake, R., Cologna, S., Howell, O., Clench, M., Wang, Y., & Griffiths, W. (2020). Visualising Cholesterol in Brain by On-Tissue Derivatisation and Quantitative Mass Spectrometry Imaging. bioRxiv
https://doi.org/10.1101/2020.11.06.369447, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60125
Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Skibinski, D., Howell, O., Wang, Y., & Griffiths, W. (2020). Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism. Proceedings of the National Academy of Sciences, 117(11), 5749-5760.
https://doi.org/10.1073/pnas.1917421117, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53792
Yutuc, E., Griffiths, W., Yutuc, E., Abdel-Khalik, J., Crick, P., Hearn, T., Dickson, A., Bigger, B., Wu, T., Goenka, A., Ghosh, A., Jones, S., Covey, D., Ory, D., & Wang, Y. (2019). Metabolism of Non-Enzymatically Derived Oxysterols: Clues from sterol metabolic disorders. Free Radical Biology and Medicine
https://doi.org/10.1016/j.freeradbiomed.2019.04.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50061
Yutuc, E., Crick, P., Yutuc, E., Abdel-Khalik, J., Saeed, A., Betsholtz, C., Genove, G., Björkhem, I., Wang, Y., & Griffiths, W. (2019). Formation and metabolism of oxysterols and cholestenoic acids found in the mouse circulation: Lessons learnt from deuterium-enrichment experiments and the CYP46A1 transgenic mouse. The Journal of Steroid Biochemistry and Molecular Biology, 195, 105475
https://doi.org/10.1016/j.jsbmb.2019.105475, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51994
Griffiths, W., Abdel-Khalik, J., Yutuc, E., Roman, G., Warner, M., Gustafsson, J., & Wang, Y. (2019). Concentrations of bile acid precursors in cerebrospinal fluid of Alzheimer's disease patients. Free Radical Biology and Medicine, 134, 42-52.
https://doi.org/10.1016/j.freeradbiomed.2018.12.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48010
Meljon, A., Crick, P., Yutuc, E., Yau, J., Seckl, J., Theofilopoulos, S., Arenas, E., Wang, Y., & Griffiths, W. (2019). Mining for Oxysterols in Cyp7b1−/− Mouse Brain and Plasma: Relevance to Spastic Paraplegia Type 5. Biomolecules, 9(4), 149
https://doi.org/10.3390/biom9040149, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50029
Yutuc, E., Abdel-Khalik, J., Crick, P., Yutuc, E., Wang, Y., & Griffiths, W. (2019). Developing an Enzyme-Assisted Derivatization Method for Analysis of C27 Bile Alcohols and Acids by Electrospray Ionization-Mass Spectrometry. Molecules, 24(3), 597
https://doi.org/10.3390/molecules24030597, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48910
Theofilopoulos, S., Oliveira, W., Yang, S., Yutuc, E., Saeed, A., Abdel-Khalik, J., Ullgren, A., Cedazo-Minguez, A., Björkhem, I., Wang, Y., Griffiths, W., & Arenas, E. (2019). 24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. Journal of Biological Chemistry, 294(11), 4169-4176.
https://doi.org/10.1074/jbc.ra118.005639, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48277
http://dx.doi.org/10.1074/jbc.ra118.005639
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Abdel-Khalik, J., Crick, P., Yutuc, E., DeBarber, A., Duell, P., Steiner, R., Laina, I., Wang, Y., & Griffiths, W. (2018). Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma. Biochimie
https://doi.org/10.1016/j.biochi.2018.06.020, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa40916
Griffiths, W., Gilmore, I., Yutuc, E., Abdel-Khalik, J., Crick, P., Hearn, T., Dickson, A., Bigger, B., Wu, T., Goenka, A., Ghosh, A., Jones, S., & Wang, Y. (2018). Identification of unusual oxysterols and bile acids with 7-oxo or 3β,5α,6β-trihydroxy functions in human plasma by charge-tagging mass spectrometry with multistage fragmentation. Journal of Lipid Research, 59(6), 1058-1070.
https://doi.org/10.1194/jlr.d083246, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa39367
Griffiths, W., Hearn, T., Crick, P., Abdel-Khalik, J., Dickson, A., Yutuc, E., & Wang, Y. (2017). Charge-tagging liquid chromatography–mass spectrometry methodology targeting oxysterol diastereoisomers. Chemistry and Physics of Lipids, 207, 69-80.
https://doi.org/10.1016/j.chemphyslip.2017.04.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32978
Griffiths, W., Abdel-Khalik, J., Yutuc, E., Morgan, A., Gilmore, I., Hearn, T., & Wang, Y. (2017). Cholesterolomics: An update. Analytical Biochemistry
https://doi.org/10.1016/j.ab.2017.01.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31729
Abdel-Khalik, J., Yutuc, E., Crick, P., Gustafsson, J., Warner, M., Roman, G., Talbot, K., Gray, E., Griffiths, W., Turner, M., & Wang, Y. (2017). Defective cholesterol metabolism in amyotrophic lateral sclerosis. Journal of Lipid Research, jlr.P071639
https://doi.org/10.1194/jlr.P071639, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa30987
Robertson, K., Hsieh, W., Forster, T., Blanc, M., Lu, H., Crick, P., Yutuc, E., Watterson, S., Martin, K., Griffiths, S., Enright, A., Yamamoto, M., Pradeepa, M., Lennox, K., Behlke, M., Talbot, S., Haas, J., Dölken, L., Griffiths, W...., & Ghazal, P. (2016). An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. PLOS Biology, 14(3), e1002364
https://doi.org/10.1371/journal.pbio.1002364, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa27043
Griffiths, W., Abdel-Khalik, J., Crick, P., Yutuc, E., & Wang, Y. (2016). New methods for analysis of oxysterols and related compounds by LC–MS. The Journal of Steroid Biochemistry and Molecular Biology, 162, 4-26.
https://doi.org/10.1016/j.jsbmb.2015.11.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25192
Griffiths, W., Abdel-Khalik, J., Hearn, T., Yutuc, E., Morgan, A., Wang, Y., Griffiths, W., Wang, Y., Morgan, A., & Yutuc, E. (2016). Current trends in oxysterol research. Biochemical Society Transactions, 44(2), 652-658.
https://doi.org/10.1042/BST20150255, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa27117

Penodau Llyfrau

Griffiths, W., Yutuc, E., & Wang, Y. (2023). Mass Spectrometry Imaging of Cholesterol and Oxysterols. Implication of Oxysterols and Phytosterols in Aging and Human Diseases (pp. 73-87). Springer International Publishing
https://doi.org/10.1007/978-3-031-43883-7_5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64898
Griffiths, W., Yutuc, E., Davies, D., Dickson, A., Angelini, R., Assad, D., Frache, G., & Wang, Y. (2020). CHAPTER 1. Lipidomics Basics. New Developments in Mass Spectrometry (pp. 1-24). Royal Society of Chemistry
https://doi.org/10.1039/9781788013109-00001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53525

Cynnyrch Ymchwil Arall

Griffiths, W., Yutuc, E., Ali Asgari, M., & Wang, Y. (2023). Role of Bile Acid Pathway Intermediates in Pathology of CTX
https://doi.org/10.5281/zenodo.10050199, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64910
Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Skibinski, D., Howell, O., Wang, Y., & Griffiths, W. (2019). Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism
https://doi.org/10.1101/450973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53208
Griffiths, W., Yutuc, E., Angelini, R., Baumert, M., Mast, N., Pikuleva, I., Newton, J., Clench, M., Howell, O., & Wang, Y. (2018). Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation-Robotic Liquid Micro-Extraction Surface Analysis-Liquid Chromatography Mass Spectrometry
https://doi.org/10.1101/450973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48402
Griffiths, W., Abdel-Khalik, J., Crick, P., Yutuc, E., Ogundare, M., Bigger, B., Morris, A., Shackleton, C., Clayton, P., Iida, T., Sircar, R., Marschall, H., Sjoval, J., Bjorkhem, I., Rohatgi, R., & Wang, Y. (2018). Bile Acid Biosynthesis Avoiding Cholesterol
https://doi.org/10.1101/385419, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48403
Yutuc, E. (2017). Analysis of Sterols in Human T Helper Cells
https://doi.org/10.23889/SUthesis.60641, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60641

Modiwlau a Addysgir

PM-280 Skills for Researchers

The modern scientist graduate is expected to have gained extensive laboratory-based experience and this is certainly essential for any student intent on pursuing a research-based career. Skills for Researchers provides students with the opportunity to acquire the core skills to be able to conduct, analyse, interpret and present scientific experiments. A proportion of the module will be spent in the laboratory gaining hands-on experience. Skills acquired in this module will form the basis for future experimental work, including the final year project. Students will have the opportunity to provide feedback to peers and engage with a variety of online learning materials.
PM-344 Capstone Project

The aim of this module is to provide a capstone experience to students¿ learning, through participating in their own enquiry-based research project, with guidance from an academic supervisor. The project may be laboratory or non-laboratory based, but it will always involve a research question that is drawn from the literature, and focused on a topic relevant to the life sciences. It will ask a research question and involve the critical analysis of research findings. Students will refine their oral and written communication skills to a graduate level through an oral presentation and dissertation on their research findings and conclusions.
PMLM22 Laboratory Measurement Techniques for Medical Sciences

This module will enable students to understand the basic and advanced concepts of laboratory and measurement techniques used in medical sciences, how they may be applied with relevant methods of detection, gain `hands on¿ problem solving experience and strategic method development for complete bioanalysis according to target molecule characteristics and method application.