Yr Athro David Lamb

Athro, Biomedical Sciences

+44 (0) 1792 602178

d.c.lamb@abertawe.ac.uk

Swyddfa Academaidd - 505
Pumed Llawr - Microbiology
Y Sefydliad Gwyddor Bywyd 1
Campws Singleton

Ar gael ar gyfer Goruchwyliaeth Ôl-raddedig

Dolenni Ymchwil

https://orcid.org/0000-0001-5446-2997

Trosolwg

Enillodd David BSc mewn Ffarmacoleg o Brifysgol Lerpwl a Doethuriaeth mewn Bioleg/Biocemeg Foleciwlaidd o Brifysgol Sheffield. Ymunodd David ag Ysgol Feddygaeth Prifysgol Abertawe fel Darllenydd yn 2004 ac fe'i penodwyd i Gadair bersonol yn 2005. Mae’n Gymrawd y Gymdeithas Gemeg Frenhinol, yn Gymrawd y Gymdeithas Fioleg Frenhinol, yn Gymrawd y Linnean Society, ac yn Ysgolor Fulbright. Dyfarnwyd DSc iddo o Brifysgol Abertawe yn 2011. Mae wedi gweithio ar fioamrywiaeth cytocrom P450 ers 25 a mwy o flynyddoedd. Mae wedi treulio cyfnod helaeth yn astudio genynnau/ensymau P450 fel Cymrawd gwadd i labordai yn yr UDA, gan gynnwys Vanderbilt University Medical School a Woods Hole Oceanographic Institute (WHOI), Human Health and Ocean Sciences. Mae wedi cael cymorth ymchwil (Prif Ymchwilydd a/neu Gyd-ymchwilydd) gan y Cyngor Ymchwil Biotechnoleg a Gwyddorau Biolegol (BBSRC), y Sefydliad Cenedlaethol dros Iechyd, Cyngor Ymchwil yr Amgylchedd Naturiol (NERC), Ymddiriedolaeth Wellcome, Ymddiriedolaeth Leverhulme, y Nuffield Foundation a'r Gymdeithas Frenhinol. Yn 2016/17 dyfarnwyd Ysgoloriaeth Fulbright y DU-UDA iddo, sy’n ysgoloriaeth o fri.

Meysydd Arbenigedd

Biocemeg
Bioleg Foleciwlaidd
Microbioleg
Tocsicoleg
Esblygiad

Uchafbwyntiau Gyrfa

Diddordebau Addysgu

Mae David yn addysgu myfyrwyr israddedig ac ôl-raddedig ym meysydd biocemeg a bioleg foleciwlaidd.  

Ymchwil Prif Wobrau Cydweithrediadau

Cyhoeddiadau

Uchafbwyntiau Ymchwil

Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
http://dx.doi.org/10.1039/d4ob00178h
Padayachee, T., Lamb, D., Nelson, D., & Syed, K. (2023). Structure–Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family. Biomolecules, 13(12), 1733
https://doi.org/10.3390/biom13121733, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66029
http://dx.doi.org/10.3390/biom13121733
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Irvine, S., Oklobia, O., Jones, S., Lamb, D., Kartopu, G., Lu, D., & Xiong, G. (2023). Creating metal saturated growth in MOCVD for CdTe solar cells. Journal of Crystal Growth, 607, 127124
https://doi.org/10.1016/j.jcrysgro.2023.127124, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62584
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
http://dx.doi.org/10.1016/j.jinorgbio.2023.112241
Brun, N., Salanga, M., Mora-Zamorano, F., Lamb, D., Goldstone, J., & Stegeman, J. (2021). Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), 23892
https://doi.org/10.1038/s41598-021-03068-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62080
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200
Aherfi, S., Belhaouari, D., Pinault, L., Baudoin, J., Decloquement, P., Abrahao, J., Colson, P., Levasseur, A., Lamb, D., Chabriere, E., Raoult, D., & Scola, B. (2022). Incomplete tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: is it still a virus?. The ISME Journal, 16(3), 695-704.
https://doi.org/10.1038/s41396-021-01117-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57909
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Lemaire, B., Kubota, A., O'Meara, C., Lamb, D., Tanguay, R., Goldstone, J., & Stegeman, J. (2016). Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders. Toxicology and Applied Pharmacology, 296, 73-84.
https://doi.org/10.1016/j.taap.2016.02.001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29840
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Hargrove, T., Wawrzak, Z., Lamb, D., Guengerich, F., & Lepesheva, G. (2015). Structure-Functional Characterization of Cytochrome P450 Sterol 14α-Demethylase (CYP51B) fromAspergillus fumigatusand Molecular Basis for the Development of Antifungal Drugs. Journal of Biological Chemistry, 290(39), 23916-23934.
https://doi.org/10.1074/jbc.M115.677310, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29844
http://www.jbc.org/content/290/39/23916.full.pdf
Morrison, A., Goldstone, J., Lamb, D., Kubota, A., Lemaire, B., & Stegeman, J. (2013). Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14α-demethylase. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(6), 1825-1836.
https://doi.org/10.1016/j.bbagen.2013.12.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31515
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118742/pdf/nihms584470.pdf
Lemaire, B., Karchner, S., Goldstone, J., Lamb, D., Drazen, J., Rees, J., Hahn, M., & Stegeman, J. (2018). Molecular adaptation to high pressure in cytochrome P450 1A and aryl hydrocarbon receptor systems of the deep-sea fish Coryphaenoides armatus. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
https://doi.org/10.1016/j.bbapap.2017.06.026, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34752
Goldstone, J., Sundaramoorthy, M., Zhao, B., Waterman, M., Stegeman, J., & Lamb, D. (2016). Genetic and structural analyses of cytochrome P450 hydroxylases in sex hormone biosynthesis: Sequential origin and subsequent coevolution. Molecular Phylogenetics and Evolution, 94, 676-687.
https://doi.org/10.1016/j.ympev.2015.09.012, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29843

Holl Ymchwil

Erthyglau Cylchgronau

Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
http://dx.doi.org/10.1039/d4ob00178h
Padayachee, T., Lamb, D., Nelson, D., & Syed, K. (2023). Structure–Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family. Biomolecules, 13(12), 1733
https://doi.org/10.3390/biom13121733, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66029
http://dx.doi.org/10.3390/biom13121733
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
http://dx.doi.org/10.1016/j.jinorgbio.2023.112241
Irvine, S., Oklobia, O., Jones, S., Lamb, D., Kartopu, G., Lu, D., & Xiong, G. (2023). Creating metal saturated growth in MOCVD for CdTe solar cells. Journal of Crystal Growth, 607, 127124
https://doi.org/10.1016/j.jcrysgro.2023.127124, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62584
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Aherfi, S., Belhaouari, D., Pinault, L., Baudoin, J., Decloquement, P., Abrahao, J., Colson, P., Levasseur, A., Lamb, D., Chabriere, E., Raoult, D., & Scola, B. (2022). Incomplete tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: is it still a virus?. The ISME Journal, 16(3), 695-704.
https://doi.org/10.1038/s41396-021-01117-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57909
Brun, N., Salanga, M., Mora-Zamorano, F., Lamb, D., Goldstone, J., & Stegeman, J. (2021). Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), 23892
https://doi.org/10.1038/s41598-021-03068-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62080
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Lemaire, B., Karchner, S., Goldstone, J., Lamb, D., Drazen, J., Rees, J., Hahn, M., & Stegeman, J. (2018). Molecular adaptation to high pressure in cytochrome P450 1A and aryl hydrocarbon receptor systems of the deep-sea fish Coryphaenoides armatus. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
https://doi.org/10.1016/j.bbapap.2017.06.026, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34752
Lemaire, B., Kubota, A., O'Meara, C., Lamb, D., Tanguay, R., Goldstone, J., & Stegeman, J. (2016). Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders. Toxicology and Applied Pharmacology, 296, 73-84.
https://doi.org/10.1016/j.taap.2016.02.001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29840
Goldstone, J., Sundaramoorthy, M., Zhao, B., Waterman, M., Stegeman, J., & Lamb, D. (2016). Genetic and structural analyses of cytochrome P450 hydroxylases in sex hormone biosynthesis: Sequential origin and subsequent coevolution. Molecular Phylogenetics and Evolution, 94, 676-687.
https://doi.org/10.1016/j.ympev.2015.09.012, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29843
Hargrove, T., Wawrzak, Z., Lamb, D., Guengerich, F., & Lepesheva, G. (2015). Structure-Functional Characterization of Cytochrome P450 Sterol 14α-Demethylase (CYP51B) fromAspergillus fumigatusand Molecular Basis for the Development of Antifungal Drugs. Journal of Biological Chemistry, 290(39), 23916-23934.
https://doi.org/10.1074/jbc.M115.677310, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29844
http://www.jbc.org/content/290/39/23916.full.pdf
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Morrison, A., Goldstone, J., Lamb, D., Kubota, A., Lemaire, B., & Stegeman, J. (2013). Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14α-demethylase. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(6), 1825-1836.
https://doi.org/10.1016/j.bbagen.2013.12.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31515
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118742/pdf/nihms584470.pdf
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200

Goruchwyliaeth

Untitled (cyfredol)

PhD

Goruchwyliwr arall: Prof Steven Kelly
Microbial Cytochrome P450 and Azole Antifungal Resistance (cyfredol)

PhD

Goruchwyliwr arall: Dr Claire Price

Modiwlau a Addysgir

PM-251 Microbial Molecular Genetics

The control systems governing gene expression in prokaryotic cells are explored and the molecular basis for gene regulation introduced. Practical skills in obtaining and analysing data on a bacterial system are developed. The module then assesses the structure of the bacterial genome and how genetic analysis can be used to investigate structure and function of individual components of that genome. Specific examples explored in depth are the genetics of recombination and DNA repair. The technologies used for functional genomic analysis of fully sequenced bacteria are described with reference to analysis of human pathogens. Finally, the module includes examples of synthetic biology applied to bacteria.
PM-344 Capstone Project

The aim of this module is to provide a capstone experience to students¿ learning, through participating in their own enquiry-based research project, with guidance from an academic supervisor. The project may be laboratory or non-laboratory based, but it will always involve a research question that is drawn from the literature, and focused on a topic relevant to the life sciences. It will ask a research question and involve the critical analysis of research findings. Students will refine their oral and written communication skills to a graduate level through an oral presentation and dissertation on their research findings and conclusions.
PMGM12 Omics Techniques and their Application to Genomic Medicine

This module explores the state of the art genomics techniques used for DNA sequencing (e.g. targeted approaches, whole exome and whole genome sequencing) and RNA sequencing. This module will include introductory bioinformatics approaches required for the analysis of genomic data, which will provide a solid foundation for the Bioinformatics module. The module will also cover the use of array based methodologies and RNA sequencing in estimating levels of protein expression, micro RNAs and gene manipulation technologies. A comprehensive introduction to metabolomics and proteomics, which are important for the functional interpretation of genomic data and discovery of disease biomarkers will also be included.
PMGM17 Dissertation Project

The module is for students to build on their previous knowledge, skills and experience by undertaking a laboratory based or suitable non-laboratory based project on a specific subject related to genomics.

Yr Athro David Lamb

Rhif ffôn

Cyfeiriad ebost

Dolenni Ymchwil

Trosolwg

Meysydd Arbenigedd

Uchafbwyntiau Gyrfa

Cyhoeddiadau

Uchafbwyntiau Ymchwil

Holl Ymchwil

Erthyglau Cylchgronau

Goruchwyliaeth

Untitled (cyfredol)

Microbial Cytochrome P450 and Azole Antifungal Resistance (cyfredol)

Modiwlau a Addysgir

PM-251 Microbial Molecular Genetics

PM-344 Capstone Project

PMGM12 Omics Techniques and their Application to Genomic Medicine

PMGM17 Dissertation Project