Professor Steven Kelly

Professor Emeritus (Human & Health Sciences), Medicine Health and Life Science

s.l.kelly@swansea.ac.uk

Basic Welsh Speaker

Available For Postgraduate Supervision

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About

Professor Steven Kelly obtained his BSc (hons) in Genetics from Swansea University in 1978 and a PhD in yeast genetics in 1982 also from Swansea University.  In 2011 he received his DSc from Swansea University and also worked as Lecturer/Senior Lecturer in the Krebs Institute in Sheffield University (1983-98) and as lead professor for molecular biology at Aberystwyth University (1998-2004) where he established the Wolfson Laboratory for Cytochrome P450 Biodiversity.  In 2004 he returned to Swansea University’s new Medical School as lead for research.

Professor Kelly is a Fellow of the Institute of Welsh Affairs, Royal Society of Biology, Royal Society of Chemistry and the Learned Society of Wales. In 2016 he was awarded the George Schroepfer Medal from the American Oil Chemist’s Society for distinguished research on lipids (sterols).  This is the foremost honour for studies on sterols. In 2014 he was First Distinguished Visiting Research Professor at the University of Tennessee in Memphis and in that year the European Regional Development Fund project BEACON with Aberystwyth and Bangor Universities won the 2014 RegioStars prize among the EU regional projects.

He is author of more than 300 publications and has been on the Advisory Board of several international symposia.  He held scientific conferences in Swansea University including the International Yeast Lipid Conference (2005), International Symposium on Cytochrome P450 Biodiversity and Biotechnology (2006), British Yeast Group (2016) and 30^th Anniversary Conference for the EPSRC UK National Mass Spectrometry Facility (2017).

Professor Kelly is Deputy-Director of the BEACON Centre of Excellence in Biorefinery, Director of the EPSRC UK National Mass Spectrometry Facility (2016-18) and besides EU and RCUK funding (BBSRC, EPSRC, MRC, NERC) has held National Institutes of Health (USA) programme RO1 support continuously in Swansea University 2004-2022.  He continues to work with multinational industries in different sectors (pharmaceuticals, agrochemicals, biotechnology, food and drink, water) and also with BEACON helping local SMEs in West Wales and the Valleys.

Areas Of Expertise

Molecular biology
Biological Chemistry
Biotechnology and Genetic Engineering
Cytochromes P450
Sterols and other cytochrome P450 substrates and inhibitors.
Antifungal drug development and resistance

Career Highlights

Research

Our research laboratory is investigating cytochrome P450 systems of bacteria, fungi, plants, protozoa, animals and in viruses. The P450 gene/enzyme system is extremely diverse, reflecting the central role it plays in xenobiotic metabolism and detoxification of diverse organic chemicals in the biosphere. As the enzymes also participate in important biosynthetic reactions such as sterol and steroid biosynthesis, inhibitors have been developed as commercially important drugs, including the azole antifungal agents, anti-protozoal therapies and the aromatase inhibiting breast cancer drugs. Other P450s may represent new targets while P450s are also involved in the biosynthesis of many of the natural products used in medicine today (e.g. erythromycin, vincristine, taxol).

The work of our laboratory includes studies on the molecular genetics and biochemistry of these systems. The approach is interdisciplinary in its approach and includes medical microbiology and mycology, microbial genetics and biotechnology, toxicology, biocatalysis, molecular biology, transcriptomics, proteomics, metabolomics and structural biology.

The Centre for Cytochrome P450 Biodiversity is always reviewing its interests, taking into account developments in post-genomic science. An ever-increasing number of cytochrome P450 genes (one per cent of a genome in some cases) are being uncovered by the DNA sequencing projects of diverse organisms and the genes generally have no known biological role. Identifying these functions in a systematic way using post-genomic technologies and bioinformatics is a challenge for the future. This will create new opportunities for basic and applied studies, besides the greater appreciation of the biology of this super family of unique proteins involving secondary metabolism, pharmacology, toxicology and biotechnology. New drugs, drug resistance and individualised responses to therapy and xenobiotic exposure are all covered in this area.

Award Highlights Collaborations

Publications

Research Highlights

Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64804
http://dx.doi.org/10.1186/s13068-017-0904-z
Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
http://dx.doi.org/10.1039/d4ob00178h
Asadzadeh, M., Alfouzan, W., Parker, J., Meis, J., Kelly, S., Joseph, L., & Ahmad, S. (2023). Molecular Characterization and Sterol Profiles Identify Nonsynonymous Mutations in ERG2 as a Major Mechanism Conferring Reduced Susceptibility to Amphotericin B in Candida kefyr. Microbiology Spectrum, 11(4)
https://doi.org/10.1128/spectrum.01474-23, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64121
http://dx.doi.org/10.1128/spectrum.01474-23
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
http://dx.doi.org/10.1016/j.jinorgbio.2023.112241
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Owen, R., Bajic, S., Kelly, S., Morris, M., & Brenton, A. (2022). Glow flow ionization mass spectrometry of small molecules. A comparison of a glow flow ionization source (‘GlowFlow’) with electrospray ionization and atmospheric pressure chemical ionization. Rapid Communications in Mass Spectrometry, 36(15)
https://doi.org/10.1002/rcm.9327, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60077
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Price, C., Warrilow, A., Rolley, N., Parker, J., Thoss, V., Kelly, D., Corcionivoschi, N., & Kelly, S. (2022). Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. PLOS ONE, 17(3), e0265227
https://doi.org/10.1371/journal.pone.0265227, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59697
Vale-Silva, L., Coste, A., Ischer, F., Parker, J., Kelly, S., Pinto, E., & Sanglard, D. (2012). Azole Resistance by Loss of Function of the Sterol Δ5,6-Desaturase Gene (ERG3) in Candida albicans Does Not Necessarily Decrease Virulence. Antimicrobial Agents and Chemotherapy, 56(4), 1960-1968.
https://doi.org/10.1128/aac.05720-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6836
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200
Hull, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation inERG11and Exhibiting Cross-Resistance to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 56(8), 4223-4232.
https://doi.org/10.1128/aac.06253-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12338
http://dx.doi.org/10.1128/aac.06253-11
Owen, R., Kelly, S., & Brenton, A. (2021). “GlowFlow”, a step towards a unified ionisation source for mass spectrometry?. Spectroscopy Europe, 33(8), 19-19.
https://doi.org/10.1255/sew.2021.a53, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58901
Ollinger, T., Vu, B., Murante, D., Parker, J., Simonicova, L., Doorley, L., Stamnes, M., Kelly, S., Rogers, P., Moye-Rowley, W., & Krysan, D. (2021). Loss-of-Function ROX1 Mutations Suppress the Fluconazole Susceptibility of upc2AΔ Mutation in Candida glabrata, Implicating Additional Positive Regulators of Ergosterol Biosynthesis. mSphere, 6(6)
https://doi.org/10.1128/msphere.00830-21, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59074
Rybak, J., Barker, K., Muñoz, J., Parker, J., Ahmad, S., Mokaddas, E., Abdullah, A., Elhagracy, R., Kelly, S., Cuomo, C., & Rogers, P. (2022). In vivo emergence of high-level resistance during treatment reveals the first identified mechanism of amphotericin B resistance in Candida auris. Clinical Microbiology and Infection, 28(6), 838-843.
https://doi.org/10.1016/j.cmi.2021.11.024, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59077
http://dx.doi.org/10.1016/j.cmi.2021.11.024
Luna-Tapia, A., Parker, J., Kelly, S., & Palmer, G. (2021). Species-Specific Differences in C-5 Sterol Desaturase Function Influence the Outcome of Azole Antifungal Exposure. Antimicrobial Agents and Chemotherapy, 65(12)
https://doi.org/10.1128/aac.01044-21, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58450
Melo, N., Moran, G., Warrilow, A., Dudley, E., Smith, S., Sullivan, D., Lamb, D., Kelly, D., Coleman, D., & Kelly, S. (2008). CYP56 (Dit2p) in Candida albicans: Characterization and Investigation of Its Role in Growth and Antifungal Drug Susceptibility. Antimicrobial Agents and Chemotherapy, 52(10), 3718-3724.
https://doi.org/10.1128/AAC.00446-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6349
Warrilow, A., Jackson, C., Parker, J., Marczylo, T., Kelly, D., Lamb, D., & Kelly, S. (2008). Identification, Characterization, and Azole-Binding Properties of Mycobacterium smegmatis CYP164A2, a Homolog of ML2088, the Sole Cytochrome P450 Gene of Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 53(3), 1157-1164.
https://doi.org/10.1128/AAC.01237-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6351
Lamb, D., Lei, L., Warrilow, A., Lepesheva, G., Mullins, J., Waterman, M., Kelly, S., & Mullins, J. (2009). The First Virally Encoded Cytochrome P450. Journal of Virology, 83(16), 8266-8269.
https://doi.org/10.1128/JVI.00289-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6352
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., Kelly, S., Parker, J., & Hull, C. (2010). A Clinical Isolate of Candida albicans with Mutations in ERG11 (Encoding Sterol 14alpha-Demethylase) and ERG5 (Encoding C22 Desaturase) Is Cross Resistant to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 54(9), 3578-3583.
https://doi.org/10.1128/AAC.00303-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6353
Warrilow, A., Melo, N., Martel, C., Parker, J., Nes, W., Kelly, S., & Kelly, D. (2010). Expression, Purification, and Characterization of Aspergillus fumigatus Sterol 14alpha-Demethylase (CYP51) Isoenzymes A and B. Antimicrobial Agents and Chemotherapy, 54(10), 4225-4234.
https://doi.org/10.1128/AAC.00316-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6354
Warrilow, A., Martel, C., Parker, J., Melo, N., Lamb, D., Nes, W., Kelly, D., Kelly, S., & Hull, C. (2010). Azole Binding Properties of Candida albicans Sterol 14alpha-Demethylase (CaCYP51). Antimicrobial Agents and Chemotherapy, 54(10), 4235-4245.
https://doi.org/10.1128/AAC.00587-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6355
Martel, C., Parker, J., Warrilow, A., Rolley, N., Kelly, S., Kelly, D., Parker, J., Hull, C., & Warrilow, A. (2010). Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14alpha-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B. Antimicrobial Agents and Chemotherapy, 54(11), 4920-4923.
https://doi.org/10.1128/AAC.00349-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6356
Lamb, D., Lei, L., Zhao, B., Yuan, H., Jackson, C., Warrilow, A., Skaug, T., Dyson, P., Dawson, E., Kelly, S., Hachey, D., & Waterman, M. (2010). Streptomyces coelicolor A3(2) CYP102 Protein, a Novel Fatty Acid Hydroxylase Encoded as a Heme Domain without an N-Terminal Redox Partner. Applied and Environmental Microbiology, 76(6), 1975-1980.
https://doi.org/10.1128/AEM.03000-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6357
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Rolley, N., Kelly, D., Kelly, S., & Hull, C. (2010). Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans. Antimicrobial Agents and Chemotherapy, 54(11), 4527-4533.
https://doi.org/10.1128/AAC.00348-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6358
Martel, C., Warrilow, A., Jackson, C., Mullins, J., Togawa, R., Parker, J., Morris, M., Donnison, I., Kelly, D., Kelly, S., Mullins, J., & Hull, C. (2010). Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans. Bioresource Technology, 101(12), 4395-4402.
https://doi.org/10.1016/j.biortech.2010.01.084, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6359
Martel, C., Parker, J., Jackson, C., Warrilow, A., Rolley, N., Greig, C., Morris, S., Donnison, I., Kelly, D., Kelly, S., & Hull, C. (2011). Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol. Bioresource Technology, 102(2), 1503-1508.
https://doi.org/10.1016/j.biortech.2010.07.099, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6360
Parker, J., Warrilow, A., Cools, H., Martel, C., Nes, W., Fraaije, B., Lucas, J., Kelly, D., Kelly, S., & Hull, C. (2010). Mechanism of Binding of Prothioconazole to Mycosphaerella graminicola CYP51 Differs from That of Other Azole Antifungals. Applied and Environmental Microbiology, 77(4), 1460-1465.
https://doi.org/10.1128/AEM.01332-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6361
Agnew, C., Warrilow, A., Burton, N., Lamb, D., Kelly, S., & Brady, R. (2011). An Enlarged, Adaptable Active Site in CYP164 Family P450 Enzymes, the Sole P450 in Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 56(1), 391-402.
https://doi.org/10.1128/AAC.05227-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6364
Parker, J., Merkamm, M., Manning, N., Pompon, D., Kelly, S., & Kelly, D. (2008). Differential Azole Antifungal Efficacies Contrasted Using a Saccharomyces cerevisiae Strain Humanized for Sterol 14 alpha-Demethylase at the Homologous Locus. Antimicrobial Agents and Chemotherapy, 52(10), 3597-3603.
https://doi.org/10.1128/AAC.00517-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6835
Mullins, J., Parker, J., Cools, H., Togawa, R., Lucas, J., Fraaije, B., Kelly, D., & Kelly, S. (2011). Molecular Modelling of the Emergence of Azole Resistance in Mycosphaerella graminicola. PLoS ONE, 6(6), e20973
https://doi.org/10.1371/journal.pone.0020973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6839
Cools, H., Mullins, J., Fraaije, B., Parker, J., Kelly, D., Lucas, J., & Kelly, S. (2011). Impact of Recently Emerged Sterol 14 alpha-Demethylase (CYP51) Variants of Mycosphaerella graminicola on Azole Fungicide Sensitivity. Applied and Environmental Microbiology, 77(11), 3830-3837.
https://doi.org/10.1128/AEM.00027-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6846
Cools, H., Parker, J., Kelly, D., Lucas, J., Fraaije, B., & Kelly, S. (2010). Heterologous Expression of Mutated Eburicol 14α-Demethylase (CYP51) Proteins of Mycosphaerella graminicola To Assess Effects on Azole Fungicide Sensitivity and Intrinsic Protein Function. Applied and Environmental Microbiology, 76(9), 2866-2872.
https://doi.org/10.1128/aem.02158-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6856
http://dx.doi.org/10.1128/aem.02158-09
Kelly, S., Kelly, D., Parker, J., & Warrilow, A. (2013). Azole Affinity of Sterol 14alpha-Demethylase (CYP51) Enzymes from Candida albicans and Homo sapiens. Antimicrobial Agents and Chemotherapy, 57(3)-1360.
https://doi.org/10.1128/AAC.02067-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14415
Fan, J., Urban, M., Parker, J., Brewer, H., Kelly, S., Hammond-Kosack, K., Fraaije, B., Liu, X., & Cools, H. (2013). Characterization of the sterol 14α-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function. New Phytologist-n/a.
https://doi.org/10.1111/NPH.12193, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14421
Warrilow, A., Hull, C., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2014). Clotrimazole as a Potent Agent for Treating the Oomycete Fish Pathogen Saprolegnia parasitica through Inhibition of Sterol 14 -Demethylase (CYP51). Applied and Environmental Microbiology, 80(19), 6154-6166.
https://doi.org/10.1128/AEM.01195-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18616
Hull, C., Loveridge, J., Rolley, N., Donnison, I., Kelly, S., & Kelly, D. (2014). Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock. Biotechnology for Biofuels, 7(1), 133
https://doi.org/10.1186/s13068-014-0133-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18617
Warrilow, A., Hull, C., Parker, J., Garvey, E., Hoekstra, W., Moore, W., Schotzinger, R., Kelly, D., Kelly, S., & Hull, C. (2014). The Clinical Candidate VT-1161 Is a Highly Potent Inhibitor of Candida albicans CYP51 but Fails To Bind the Human Enzyme. Antimicrobial Agents and Chemotherapy, 58(12), 7121-7127.
https://doi.org/10.1128/AAC.03707-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18693
Parker, J., Warrilow, A., Price, C., Mullins, J., Kelly, D., & Kelly, S. (2014). Resistance to antifungals that target CYP51. Journal of Chemical Biology, 7(4), 143-161.
https://doi.org/10.1007/s12154-014-0121-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18694
Hull, C., Loveridge, J., Donnison, I., Kelly, D., & Kelly, S. (2014). Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept. AMB Express, 4(1), 64
https://doi.org/10.1186/s13568-014-0064-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18763
Salvage, R., Hull, C., Kelly, D., & Kelly, S. (2014). Use of 70% alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms. Future Microbiology, 9(10), 1123-1130.
https://doi.org/10.2217/fmb.14.73, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19624
Price, C., Warrilow, A., Parker, J., Mullins, J., Nes, W., Kelly, D., Kelly, S., & Schottel, J. (2015). Novel Substrate Specificity and Temperature-Sensitive Activity of Mycosphaerella graminicola CYP51 Supported by the Native NADPH Cytochrome P450 Reductase. Applied and Environmental Microbiology, 81(10), 3379-3386.
https://doi.org/10.1128/AEM.03965-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20704
Price, C., Parker, J., Warrilow, A., Kelly, D., & Kelly, S. (2015). Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens. Pest Management Science, n/a-n/a.
https://doi.org/10.1002/ps.4029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20922
Derbyshire, M., Michaelson, L., Parker, J., Kelly, S., Thacker, U., Powers, S., Bailey, A., Hammond-Kosack, K., Courbot, M., & Rudd, J. (2015). Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence. Fungal Genetics and Biology
https://doi.org/10.1016/j.fgb.2015.05.008, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa22091
Berkow, E., Manigaba, K., Parker, J., Barker, K., Kelly, S., & Rogers, P. (2015). Multidrug Transporters and Alterations in Sterol Biosynthesis Contribute to Azole Antifungal Resistance in Candida parapsilosis. Antimicrobial Agents and Chemotherapy, 59(10), 5942-5950.
https://doi.org/10.1128/aac.01358-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa22523
http://dx.doi.org/10.1128/aac.01358-15
Warrilow, A., Parker, J., Price, C., Nes, W., Kelly, S., & Kelly, D. (2015). In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus. Antimicrobial Agents and Chemotherapy, 59(12), 7771-7778.
https://doi.org/10.1128/aac.01806-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa23614
http://dx.doi.org/10.1128/aac.01806-15
Warrilow, A., Parker, J., Price, C., Nes, W., Garvey, E., Hoekstra, W., Schotzinger, R., Kelly, D., & Kelly, S. (2016). The Investigational Drug VT-1129 Is a Highly Potent Inhibitor of Cryptococcus Species CYP51 but Only Weakly Inhibits the Human Enzyme. Antimicrobial Agents and Chemotherapy, 60(8), 4530-4538.
https://doi.org/10.1128/AAC.00349-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29154
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Colley, T., Alanio, A., Kelly, S., Sehra, G., Kizawa, Y., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Anderson-Dring, L., Nakaoki, T., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J., Cooke, M., Bretagne, S...., & Rapeport, G. (2017). In vitro and in vivo antifungal profile of a novel and long acting inhaled azole, PC945, on Aspergillus fumigatus infection. Antimicrobial Agents and Chemotherapy, AAC.02280-16
https://doi.org/10.1128/AAC.02280-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32189
Warrilow, A., Parker, J., Price, C., Garvey, E., Hoekstra, W., Schotzinger, R., Wiederhold, N., Nes, W., Kelly, D., & Kelly, S. (2017). The Tetrazole VT-1161 Is a Potent Inhibitor of Trichophyton rubrum through Its Inhibition of T. rubrum CYP51. Antimicrobial Agents and Chemotherapy, 61(7), e00333-17
https://doi.org/10.1128/AAC.00333-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34176
Warrilow, A., Price, C., Parker, J., Rolley, N., Smyrniotis, C., Hughes, D., Thoss, V., Nes, W., Kelly, D., Holman, T., & Kelly, S. (2016). Azole Antifungal Sensitivity of Sterol 14α-Demethylase (CYP51) and CYP5218 from Malassezia globosa. Scientific Reports, 6(1)
https://doi.org/10.1038/srep27690, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34335
Butts, A., DeJarnette, C., Peters, T., Parker, J., Kerns, M., Eberle, K., Kelly, S., & Palmer, G. (2017). Target Abundance-Based Fitness Screening (TAFiS) Facilitates Rapid Identification of Target-Specific and Physiologically Active Chemical Probes. mSphere, 2(5), e00379-17
https://doi.org/10.1128/mSphere.00379-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36657
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37567
Colley, T., Sehra, G., Chowdhary, A., Alanio, A., Kelly, S., Kizawa, Y., Armstrong-James, D., Fisher, M., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Nishimoto, Y., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J...., & Rapeport, G. (2018). In Vitro and In Vivo Efficacy of a Novel and Long-Acting Fungicidal Azole, PC1244, on Aspergillus fumigatus Infection. Antimicrobial Agents and Chemotherapy, 62(5), AAC.01941-17
https://doi.org/10.1128/aac.01941-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa38876
http://dx.doi.org/10.1128/aac.01941-17
Zhou, W., Warrilow, A., Thomas, C., Ramos, E., Parker, J., Price, C., Vanderloop, B., Fisher, P., Loftis, M., Kelly, D., Kelly, S., & Nes, W. (2018). Functional importance for developmental regulation of sterol biosynthesis in Acanthamoeba castellanii. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1863(10), 1164-1178.
https://doi.org/10.1016/j.bbalip.2018.07.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa41148
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment, 656, 531-539.
https://doi.org/10.1016/j.scitotenv.2018.11.393, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46109
Warrilow, A., Nishimoto, A., Parker, J., Price, C., Flowers, S., Kelly, D., Rogers, P., & Kelly, S. (2019). The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions. Antimicrobial Agents and Chemotherapy, 63(5)
https://doi.org/10.1128/aac.02586-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48946
Plácido, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology, 196, 106163
https://doi.org/10.1016/j.fuproc.2019.106163, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51273
Kannan, A., Asner, S., Trachsel, E., Kelly, S., Parker, J., & Sanglard, D. (2019). Comparative Genomics for the Elucidation of Multidrug Resistance in Candida lusitaniae. mBio, 10(6)
https://doi.org/10.1128/mbio.02512-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53151
Furukawa, T., Rhijn, N., Fraczek, M., Gsaller, F., Davies, E., Carr, P., Gago, S., Fortune-Grant, R., Rahman, S., Gilsenan, J., Houlder, E., Kowalski, C., Raj, S., Paul, S., Cook, P., Parker, J., Kelly, S., Cramer, R., Latgé, J...., & Bromley, M. (2020). The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus. Nature Communications, 11(1)
https://doi.org/10.1038/s41467-019-14191-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53507
http://dx.doi.org/10.1038/s41467-019-14191-1
Morgan, S., Pilia, N., Hewitt, M., Moses, R., Moseley, R., Lewis, P., Morrison, P., Kelly, S., Parker, J., Whitaker, D., Quantock, A., & Heard, C. (2020). Controlled in vitro delivery of voriconazole and diclofenac to the cornea using contact lenses for the treatment of Acanthamoeba keratitis. International Journal of Pharmaceutics, 579, 119102
https://doi.org/10.1016/j.ijpharm.2020.119102, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53795
Rybak, J., Muñoz, J., Barker, K., Parker, J., Esquivel, B., Berkow, E., Lockhart, S., Gade, L., Palmer, G., White, T., Kelly, S., Cuomo, C., & Rogers, P. (2020). Mutations in TAC1B: a Novel Genetic Determinant of Clinical Fluconazole Resistance in Candida auris. mBio, 11(3)
https://doi.org/10.1128/mbio.00365-20, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54319
Binjubair, F., Parker, J., Warrilow, A., Puri, K., Braidley, P., Tatar, E., Kelly, S., Kelly, D., & Simons, C. (2020). Small‐Molecule Inhibitors Targeting Sterol 14α‐Demethylase (CYP51): Synthesis, Molecular Modelling and Evaluation Against Candida albicans. ChemMedChem, 15(14), 1294-1309.
https://doi.org/10.1002/cmdc.202000250, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54878
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Owen, R., Kelly, S., & Brenton, A. (2021). Towards a universal ion source: Glow Flow mass spectrometry. International Journal of Mass Spectrometry, 466, 116603
https://doi.org/10.1016/j.ijms.2021.116603, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57001
Rybak, J., Ge, W., Wiederhold, N., Parker, J., Kelly, S., Rogers, P., & Fortwendel, J. (2019). Mutations in hmg1, Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus. mBio, 10(2)
https://doi.org/10.1128/mbio.00437-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49873
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Plácido, J., López, S., Meissner, K., Kelly, D., Kelly, S., & Placido Escobar, J. (2019). Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment, 688, 751-761.
https://doi.org/10.1016/j.scitotenv.2019.06.342, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50904
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment, 689, 10-20.
https://doi.org/10.1016/j.scitotenv.2019.06.390, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50905
Warrilow, A., Parker, J., Price, C., Rolley, N., Nes, W., Kelly, D., & Kelly, S. (2019). Isavuconazole and voriconazole inhibition of sterol 14α-demethylases (CYP51) from Aspergillus fumigatus and Homo sapiens. International Journal of Antimicrobial Agents
https://doi.org/10.1016/j.ijantimicag.2019.07.011, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51096
Nishimoto, A., Wiederhold, N., Flowers, S., Zhang, Q., Kelly, S., Morschhäuser, J., Yates, C., Hoekstra, W., Schotzinger, R., Garvey, E., & Rogers, P. (2019). In Vitro Activities of the Novel Investigational Tetrazoles VT-1161 and VT-1598 Compared to the Triazole Antifungals against Azole-Resistant Strains and Clinical Isolates of Candida albicans. Antimicrobial Agents and Chemotherapy, 63(6)
https://doi.org/10.1128/aac.00341-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49785
http://dx.doi.org/10.1128/aac.00341-19
Ahmad, S., Joseph, L., Parker, J., Asadzadeh, M., Kelly, S., Meis, J., & Khan, Z. (2018). ERG6 and ERG2 Are Major Targets Conferring Reduced Susceptibility to Amphotericin B in Clinical Candida glabrata Isolates in Kuwait. Antimicrobial Agents and Chemotherapy, 63(2), e01900-18
https://doi.org/10.1128/AAC.01900-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45903
Mejía, A., Luna, D., Fernández, F., Barrios-González, J., H. Gutierrez, L., Reyes, A., Absalón, A., & Kelly, S. (2018). Improving rifamycin production in Amycolatopsis mediterranei by expressing a Vitreoscilla hemoglobin (vhb) gene fused to a cytochrome P450 monooxygenase domain. 3 Biotech, 8(11)
https://doi.org/10.1007/s13205-018-1472-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45408
Luna-Tapia, A., Willems, H., Parker, J., Tournu, H., Barker, K., Nishimoto, A., Rogers, P., Kelly, S., Peters, B., & Palmer, G. (2018). Loss of Upc2p-Inducible ERG3 Transcription Is Sufficient To Confer Niche-Specific Azole Resistance without Compromising Candida albicans Pathogenicity. mBio, 9(3), e00225-18
https://doi.org/10.1128/mBio.00225-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa40336
Sewell, T., Hawkins, N., Stotz, H., Huang, Y., Kelly, S., Kelly, D., Fraaije, B., & Fitt, B. (2017). Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase. Scientific Reports, 7(1)
https://doi.org/10.1038/s41598-017-15545-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36850
Rybak, J., Dickens, C., Parker, J., Caudle, K., Manigaba, K., Whaley, S., Nishimoto, A., Luna-Tapia, A., Roy, S., Zhang, Q., Barker, K., Palmer, G., Sutter, T., Homayouni, R., Wiederhold, N., Kelly, S., & Rogers, P. (2017). Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis. Antimicrobial Agents and Chemotherapy, 61(9), AAC.00651-17
https://doi.org/10.1128/aac.00651-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34548
http://dx.doi.org/10.1128/aac.00651-17
McCourt, P., Liu, H., Parker, J., Gallo-Ebert, C., Donigan, M., Bata, A., Giordano, C., Kelly, S., Nickels, J., & Parker, J. (2016). Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence. G3 Genes|Genomes|Genetics, 6(11)
https://doi.org/10.1534/g3.116.033969, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31346
Hawkins, N., Cools, H., Sierotzki, H., Shaw, M., Knogge, W., Kelly, S., Kelly, D., & Fraaije, B. (2014). Paralog Re-Emergence: A Novel, Historically Contingent Mechanism in the Evolution of Antimicrobial Resistance. Molecular Biology and Evolution, 31(7), 1793-1802.
https://doi.org/10.1093/molbev/msu134, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18161
http://dx.doi.org/10.1093/molbev/msu134
Carter, H., Fraaije, B., West, J., Kelly, S., Mehl, A., Shaw, M., & Cools, H. (2014). Alterations in the predicted regulatory and coding regions of the sterol 14α-demethylase gene (CYP51) confer decreased azole sensitivity in the oilseed rape pathogen Pyrenopeziza brassicae.. Mol Plant Pathol., 15(5), 513-522.
https://doi.org/10.1111/mpp.12106, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18160
Reid, E., Weynberg, K., Love, J., Isupov, M., Littlechild, J., Wilson, W., Kelly, S., Lamb, D., & Allen, M. (2013). Functional and structural characterisation of a viral cytochromeb5. FEBS Letters, 587(22), 3633-3639.
https://doi.org/10.1016/j.febslet.2013.09.035, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18159
http://dx.doi.org/10.1016/j.febslet.2013.09.035
Belič, A., Pompon, D., Monostory, K., Kelly, D., Kelly, S., & Rozman, D. (2013). An algorithm for rapid computational construction of metabolic networks: A cholesterol biosynthesis example. Computers in Biology and Medicine, 43(5), 471-480.
https://doi.org/10.1016/j.compbiomed.2013.02.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15174
http://dx.doi.org/10.1016/j.compbiomed.2013.02.017
Nie, D., Hoobler, E., Rai, G., Warrilow, A., Perry, S., Smyrniotis, C., Jadhav, A., Simeonov, A., Parker, J., Kelly, D., Maloney, D., Kelly, S., & Holman, T. (2013). Discovery of a Novel Dual Fungal CYP51/Human 5-Lipoxygenase Inhibitor: Implications for Anti-Fungal Therapy. PLoS ONE, 8(6)
https://doi.org/10.1371/journal.pone.0065928, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15178
Eddouzi, J., Parker, J., Vale-Silva, L., Coste, A., Ischer, F., Kelly, S., Manai, M., & Sanglard, D. (2013). Molecular Mechanisms of Drug Resistance in Clinical Candida Species Isolated from Tunisian Hospitals. Antimicrobial Agents and Chemotherapy, 57(7), 3182-3193.
https://doi.org/10.1128/AAC.00555-13, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15187
Cheng, Q., Lamb, D., Kelly, S., Lei, L., Guenguerich, F., & Kelly, D. (2010). Cyclization of a Cellular Dipentaenone by Streptomyces coelicolor Cytochrome P450 154A1 without Oxidation/Reduction. Journal of the American Chemical Society, 132(43), 15173-15175.
https://doi.org/10.1021/ja107801v, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9901
Higgins, J., Pinjon, E., Oltean, H., White, T., Kelly, S., Martel, C., Sulivan, D., Coleman, D., Moran, G., & Hull, C. (2012). Triclosan anatagonizes fluconazole activity against candida albicans. Journal of Dental Research, 91(1), 65-70.
https://doi.org/10.1177/0022034511425046, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10166
Zhao, B., Lei, L., Vassylyev, D., Lin, X., Cane, D., Kelly, S., Yuan, H., Lamb, D., & Waterman, M. (2009). Crystal structure of albaflavenone monoxygenase containing a moonlighting terpene synthase active site.. Journal of Biological Chemistry, 284(52), 36711-9.
https://doi.org/10.1074/jbc.M109.064683, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10330
Jackson, C., Barton, R., Clark, C., & Kelly, S. (2009). Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi. Medical Mycology, 47(6), 609-617.
https://doi.org/10.1080/13693780802385445, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10333
Regina, G., D’Auria, F., Tafi, A., Piscitelli, F., Olla, S., Caporuscio, F., Nencioni, L., Cirilli, R., Torre, F., Melo, N., Kelly, S., Lamb, D., Artico, M., Botta, M., Palamara, A., & Silvestri, R. (2008). 1-[(3-Aryloxy-3-aryl)propyl]-1H-imidazoles, New Imidazoles with Potent Activity againstCandida albicansand Dermatophytes. Synthesis, Structure−Activity Relationship, and Molecular Modeling Studies. Journal of Medicinal Chemistry, 51(13), 3841-3855.
https://doi.org/10.1021/jm800009r, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10336
Zhao, B., Lin, X., Lei, L., Lei, L., Lamb, D., Kelly, S., Waterman, M., & Cane, D. (2008). Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 203(12), 8183-9.
https://doi.org/10.1074/jbc.M710421200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10337
Moody, S., Zhao, B., Lei, L., Mullins, J., Waterman, M., Kelly, S., & Lamb, D. (2012). Investigating conservation of the albaflavenone biosynthetic pathway and CYP170 bifunctionality in streptomycetes.. FEBS J., 279(9), 1640-1649.
https://doi.org/10.1111/j.1742-4658.2011.08447.x., SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10672
Palabiyik, B., Karaer, S., Arda, N., Toker, S., Temizkan, G., Kelly, S., & Sarikaya, A. (2008). Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe. Biologia, 63(1), 450-454.
https://doi.org/10.2478/s11756-008-0069-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10676
Zhao, B., Moody, S., Hider, R., Lei, L., Kelly, S., Waterman, M., & Lamb, D. (2012). Structural Analysis of Cytochrome P450 105N1 Involved in the Biosynthesis of the Zincophore, Coelibactin. International Journal of Molecular Sciences, 13(7), 8500-8513.
https://doi.org/10.3390/ijms13078500, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13027
http://dx.doi.org/10.3390/ijms13078500
Hull, C., Bader, O., Parker, J., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Two Clinical Isolates of Candida glabrata Exhibiting Reduced Sensitivity to Amphotericin B Both Harbor Mutations inERG2. Antimicrobial Agents and Chemotherapy, 56(12), 6417-6421.
https://doi.org/10.1128/aac.01145-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13028
http://dx.doi.org/10.1128/aac.01145-12
Calabrese, E., Castellano, S., Santoriello, M., Sgherri, C., Quartacci, M., Calucci, L., Warrilow, A., Lamb, D., Kelly, S., Milite, C., Granata, I., Sbardella, G., Stefancich, G., Maresca, B., & Porta, A. (2013). Antifungal activity of azole compounds CPA18 and CPA109 against azole-susceptible and -resistant strains of Candida albicans. Journal of Antimicrobial Chemotherapy, 68(5), 1111-1119.
https://doi.org/10.1093/jac/dks506, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14005
Kelly, S. & Kelly, D. (2013). Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1612), 20120476-20120476.
https://doi.org/10.1098/rstb.2012.0476, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14006

All Research

Journal Articles

Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
http://dx.doi.org/10.1039/d4ob00178h
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
http://dx.doi.org/10.1016/j.jinorgbio.2023.112241
Asadzadeh, M., Alfouzan, W., Parker, J., Meis, J., Kelly, S., Joseph, L., & Ahmad, S. (2023). Molecular Characterization and Sterol Profiles Identify Nonsynonymous Mutations in ERG2 as a Major Mechanism Conferring Reduced Susceptibility to Amphotericin B in Candida kefyr. Microbiology Spectrum, 11(4)
https://doi.org/10.1128/spectrum.01474-23, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64121
http://dx.doi.org/10.1128/spectrum.01474-23
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Owen, R., Bajic, S., Kelly, S., Morris, M., & Brenton, A. (2022). Glow flow ionization mass spectrometry of small molecules. A comparison of a glow flow ionization source (‘GlowFlow’) with electrospray ionization and atmospheric pressure chemical ionization. Rapid Communications in Mass Spectrometry, 36(15)
https://doi.org/10.1002/rcm.9327, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60077
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Rybak, J., Barker, K., Muñoz, J., Parker, J., Ahmad, S., Mokaddas, E., Abdullah, A., Elhagracy, R., Kelly, S., Cuomo, C., & Rogers, P. (2022). In vivo emergence of high-level resistance during treatment reveals the first identified mechanism of amphotericin B resistance in Candida auris. Clinical Microbiology and Infection, 28(6), 838-843.
https://doi.org/10.1016/j.cmi.2021.11.024, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59077
http://dx.doi.org/10.1016/j.cmi.2021.11.024
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Price, C., Warrilow, A., Rolley, N., Parker, J., Thoss, V., Kelly, D., Corcionivoschi, N., & Kelly, S. (2022). Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. PLOS ONE, 17(3), e0265227
https://doi.org/10.1371/journal.pone.0265227, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59697
Ollinger, T., Vu, B., Murante, D., Parker, J., Simonicova, L., Doorley, L., Stamnes, M., Kelly, S., Rogers, P., Moye-Rowley, W., & Krysan, D. (2021). Loss-of-Function ROX1 Mutations Suppress the Fluconazole Susceptibility of upc2AΔ Mutation in Candida glabrata, Implicating Additional Positive Regulators of Ergosterol Biosynthesis. mSphere, 6(6)
https://doi.org/10.1128/msphere.00830-21, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59074
Owen, R., Kelly, S., & Brenton, A. (2021). “GlowFlow”, a step towards a unified ionisation source for mass spectrometry?. Spectroscopy Europe, 33(8), 19-19.
https://doi.org/10.1255/sew.2021.a53, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58901
Luna-Tapia, A., Parker, J., Kelly, S., & Palmer, G. (2021). Species-Specific Differences in C-5 Sterol Desaturase Function Influence the Outcome of Azole Antifungal Exposure. Antimicrobial Agents and Chemotherapy, 65(12)
https://doi.org/10.1128/aac.01044-21, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58450
Owen, R., Kelly, S., & Brenton, A. (2021). Towards a universal ion source: Glow Flow mass spectrometry. International Journal of Mass Spectrometry, 466, 116603
https://doi.org/10.1016/j.ijms.2021.116603, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57001
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Furukawa, T., Rhijn, N., Fraczek, M., Gsaller, F., Davies, E., Carr, P., Gago, S., Fortune-Grant, R., Rahman, S., Gilsenan, J., Houlder, E., Kowalski, C., Raj, S., Paul, S., Cook, P., Parker, J., Kelly, S., Cramer, R., Latgé, J...., & Bromley, M. (2020). The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus. Nature Communications, 11(1)
https://doi.org/10.1038/s41467-019-14191-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53507
http://dx.doi.org/10.1038/s41467-019-14191-1
Binjubair, F., Parker, J., Warrilow, A., Puri, K., Braidley, P., Tatar, E., Kelly, S., Kelly, D., & Simons, C. (2020). Small‐Molecule Inhibitors Targeting Sterol 14α‐Demethylase (CYP51): Synthesis, Molecular Modelling and Evaluation Against Candida albicans. ChemMedChem, 15(14), 1294-1309.
https://doi.org/10.1002/cmdc.202000250, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54878
Rybak, J., Muñoz, J., Barker, K., Parker, J., Esquivel, B., Berkow, E., Lockhart, S., Gade, L., Palmer, G., White, T., Kelly, S., Cuomo, C., & Rogers, P. (2020). Mutations in TAC1B: a Novel Genetic Determinant of Clinical Fluconazole Resistance in Candida auris. mBio, 11(3)
https://doi.org/10.1128/mbio.00365-20, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54319
Morgan, S., Pilia, N., Hewitt, M., Moses, R., Moseley, R., Lewis, P., Morrison, P., Kelly, S., Parker, J., Whitaker, D., Quantock, A., & Heard, C. (2020). Controlled in vitro delivery of voriconazole and diclofenac to the cornea using contact lenses for the treatment of Acanthamoeba keratitis. International Journal of Pharmaceutics, 579, 119102
https://doi.org/10.1016/j.ijpharm.2020.119102, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53795
Rybak, J., Ge, W., Wiederhold, N., Parker, J., Kelly, S., Rogers, P., & Fortwendel, J. (2019). Mutations in hmg1, Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus. mBio, 10(2)
https://doi.org/10.1128/mbio.00437-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49873
Warrilow, A., Parker, J., Price, C., Rolley, N., Nes, W., Kelly, D., & Kelly, S. (2019). Isavuconazole and voriconazole inhibition of sterol 14α-demethylases (CYP51) from Aspergillus fumigatus and Homo sapiens. International Journal of Antimicrobial Agents
https://doi.org/10.1016/j.ijantimicag.2019.07.011, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51096
Kannan, A., Asner, S., Trachsel, E., Kelly, S., Parker, J., & Sanglard, D. (2019). Comparative Genomics for the Elucidation of Multidrug Resistance in Candida lusitaniae. mBio, 10(6)
https://doi.org/10.1128/mbio.02512-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53151
Plácido, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology, 196, 106163
https://doi.org/10.1016/j.fuproc.2019.106163, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51273
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment, 689, 10-20.
https://doi.org/10.1016/j.scitotenv.2019.06.390, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50905
Plácido, J., López, S., Meissner, K., Kelly, D., Kelly, S., & Placido Escobar, J. (2019). Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment, 688, 751-761.
https://doi.org/10.1016/j.scitotenv.2019.06.342, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50904
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Nishimoto, A., Wiederhold, N., Flowers, S., Zhang, Q., Kelly, S., Morschhäuser, J., Yates, C., Hoekstra, W., Schotzinger, R., Garvey, E., & Rogers, P. (2019). In Vitro Activities of the Novel Investigational Tetrazoles VT-1161 and VT-1598 Compared to the Triazole Antifungals against Azole-Resistant Strains and Clinical Isolates of Candida albicans. Antimicrobial Agents and Chemotherapy, 63(6)
https://doi.org/10.1128/aac.00341-19, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49785
http://dx.doi.org/10.1128/aac.00341-19
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment, 656, 531-539.
https://doi.org/10.1016/j.scitotenv.2018.11.393, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46109
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Warrilow, A., Nishimoto, A., Parker, J., Price, C., Flowers, S., Kelly, D., Rogers, P., & Kelly, S. (2019). The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions. Antimicrobial Agents and Chemotherapy, 63(5)
https://doi.org/10.1128/aac.02586-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48946
Mejía, A., Luna, D., Fernández, F., Barrios-González, J., H. Gutierrez, L., Reyes, A., Absalón, A., & Kelly, S. (2018). Improving rifamycin production in Amycolatopsis mediterranei by expressing a Vitreoscilla hemoglobin (vhb) gene fused to a cytochrome P450 monooxygenase domain. 3 Biotech, 8(11)
https://doi.org/10.1007/s13205-018-1472-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45408
Ahmad, S., Joseph, L., Parker, J., Asadzadeh, M., Kelly, S., Meis, J., & Khan, Z. (2018). ERG6 and ERG2 Are Major Targets Conferring Reduced Susceptibility to Amphotericin B in Clinical Candida glabrata Isolates in Kuwait. Antimicrobial Agents and Chemotherapy, 63(2), e01900-18
https://doi.org/10.1128/AAC.01900-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45903
Zhou, W., Warrilow, A., Thomas, C., Ramos, E., Parker, J., Price, C., Vanderloop, B., Fisher, P., Loftis, M., Kelly, D., Kelly, S., & Nes, W. (2018). Functional importance for developmental regulation of sterol biosynthesis in Acanthamoeba castellanii. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1863(10), 1164-1178.
https://doi.org/10.1016/j.bbalip.2018.07.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa41148
Luna-Tapia, A., Willems, H., Parker, J., Tournu, H., Barker, K., Nishimoto, A., Rogers, P., Kelly, S., Peters, B., & Palmer, G. (2018). Loss of Upc2p-Inducible ERG3 Transcription Is Sufficient To Confer Niche-Specific Azole Resistance without Compromising Candida albicans Pathogenicity. mBio, 9(3), e00225-18
https://doi.org/10.1128/mBio.00225-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa40336
Colley, T., Sehra, G., Chowdhary, A., Alanio, A., Kelly, S., Kizawa, Y., Armstrong-James, D., Fisher, M., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Nishimoto, Y., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J...., & Rapeport, G. (2018). In Vitro and In Vivo Efficacy of a Novel and Long-Acting Fungicidal Azole, PC1244, on Aspergillus fumigatus Infection. Antimicrobial Agents and Chemotherapy, 62(5), AAC.01941-17
https://doi.org/10.1128/aac.01941-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa38876
http://dx.doi.org/10.1128/aac.01941-17
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64804
http://dx.doi.org/10.1186/s13068-017-0904-z
Butts, A., DeJarnette, C., Peters, T., Parker, J., Kerns, M., Eberle, K., Kelly, S., & Palmer, G. (2017). Target Abundance-Based Fitness Screening (TAFiS) Facilitates Rapid Identification of Target-Specific and Physiologically Active Chemical Probes. mSphere, 2(5), e00379-17
https://doi.org/10.1128/mSphere.00379-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36657
Sewell, T., Hawkins, N., Stotz, H., Huang, Y., Kelly, S., Kelly, D., Fraaije, B., & Fitt, B. (2017). Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase. Scientific Reports, 7(1)
https://doi.org/10.1038/s41598-017-15545-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36850
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37567
Rybak, J., Dickens, C., Parker, J., Caudle, K., Manigaba, K., Whaley, S., Nishimoto, A., Luna-Tapia, A., Roy, S., Zhang, Q., Barker, K., Palmer, G., Sutter, T., Homayouni, R., Wiederhold, N., Kelly, S., & Rogers, P. (2017). Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis. Antimicrobial Agents and Chemotherapy, 61(9), AAC.00651-17
https://doi.org/10.1128/aac.00651-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34548
http://dx.doi.org/10.1128/aac.00651-17
Warrilow, A., Parker, J., Price, C., Garvey, E., Hoekstra, W., Schotzinger, R., Wiederhold, N., Nes, W., Kelly, D., & Kelly, S. (2017). The Tetrazole VT-1161 Is a Potent Inhibitor of Trichophyton rubrum through Its Inhibition of T. rubrum CYP51. Antimicrobial Agents and Chemotherapy, 61(7), e00333-17
https://doi.org/10.1128/AAC.00333-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34176
Colley, T., Alanio, A., Kelly, S., Sehra, G., Kizawa, Y., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Anderson-Dring, L., Nakaoki, T., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J., Cooke, M., Bretagne, S...., & Rapeport, G. (2017). In vitro and in vivo antifungal profile of a novel and long acting inhaled azole, PC945, on Aspergillus fumigatus infection. Antimicrobial Agents and Chemotherapy, AAC.02280-16
https://doi.org/10.1128/AAC.02280-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32189
Warrilow, A., Price, C., Parker, J., Rolley, N., Smyrniotis, C., Hughes, D., Thoss, V., Nes, W., Kelly, D., Holman, T., & Kelly, S. (2016). Azole Antifungal Sensitivity of Sterol 14α-Demethylase (CYP51) and CYP5218 from Malassezia globosa. Scientific Reports, 6(1)
https://doi.org/10.1038/srep27690, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34335
McCourt, P., Liu, H., Parker, J., Gallo-Ebert, C., Donigan, M., Bata, A., Giordano, C., Kelly, S., Nickels, J., & Parker, J. (2016). Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence. G3 Genes|Genomes|Genetics, 6(11)
https://doi.org/10.1534/g3.116.033969, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31346
Warrilow, A., Parker, J., Price, C., Nes, W., Garvey, E., Hoekstra, W., Schotzinger, R., Kelly, D., & Kelly, S. (2016). The Investigational Drug VT-1129 Is a Highly Potent Inhibitor of Cryptococcus Species CYP51 but Only Weakly Inhibits the Human Enzyme. Antimicrobial Agents and Chemotherapy, 60(8), 4530-4538.
https://doi.org/10.1128/AAC.00349-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29154
Price, C., Parker, J., Warrilow, A., Kelly, D., & Kelly, S. (2015). Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens. Pest Management Science, n/a-n/a.
https://doi.org/10.1002/ps.4029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20922
Derbyshire, M., Michaelson, L., Parker, J., Kelly, S., Thacker, U., Powers, S., Bailey, A., Hammond-Kosack, K., Courbot, M., & Rudd, J. (2015). Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence. Fungal Genetics and Biology
https://doi.org/10.1016/j.fgb.2015.05.008, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa22091
Warrilow, A., Parker, J., Price, C., Nes, W., Kelly, S., & Kelly, D. (2015). In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus. Antimicrobial Agents and Chemotherapy, 59(12), 7771-7778.
https://doi.org/10.1128/aac.01806-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa23614
http://dx.doi.org/10.1128/aac.01806-15
Berkow, E., Manigaba, K., Parker, J., Barker, K., Kelly, S., & Rogers, P. (2015). Multidrug Transporters and Alterations in Sterol Biosynthesis Contribute to Azole Antifungal Resistance in Candida parapsilosis. Antimicrobial Agents and Chemotherapy, 59(10), 5942-5950.
https://doi.org/10.1128/aac.01358-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa22523
http://dx.doi.org/10.1128/aac.01358-15
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Price, C., Warrilow, A., Parker, J., Mullins, J., Nes, W., Kelly, D., Kelly, S., & Schottel, J. (2015). Novel Substrate Specificity and Temperature-Sensitive Activity of Mycosphaerella graminicola CYP51 Supported by the Native NADPH Cytochrome P450 Reductase. Applied and Environmental Microbiology, 81(10), 3379-3386.
https://doi.org/10.1128/AEM.03965-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20704
Warrilow, A., Hull, C., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2014). Clotrimazole as a Potent Agent for Treating the Oomycete Fish Pathogen Saprolegnia parasitica through Inhibition of Sterol 14 -Demethylase (CYP51). Applied and Environmental Microbiology, 80(19), 6154-6166.
https://doi.org/10.1128/AEM.01195-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18616
Parker, J., Warrilow, A., Price, C., Mullins, J., Kelly, D., & Kelly, S. (2014). Resistance to antifungals that target CYP51. Journal of Chemical Biology, 7(4), 143-161.
https://doi.org/10.1007/s12154-014-0121-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18694
Salvage, R., Hull, C., Kelly, D., & Kelly, S. (2014). Use of 70% alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms. Future Microbiology, 9(10), 1123-1130.
https://doi.org/10.2217/fmb.14.73, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19624
Carter, H., Fraaije, B., West, J., Kelly, S., Mehl, A., Shaw, M., & Cools, H. (2014). Alterations in the predicted regulatory and coding regions of the sterol 14α-demethylase gene (CYP51) confer decreased azole sensitivity in the oilseed rape pathogen Pyrenopeziza brassicae.. Mol Plant Pathol., 15(5), 513-522.
https://doi.org/10.1111/mpp.12106, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18160
Warrilow, A., Hull, C., Parker, J., Garvey, E., Hoekstra, W., Moore, W., Schotzinger, R., Kelly, D., Kelly, S., & Hull, C. (2014). The Clinical Candidate VT-1161 Is a Highly Potent Inhibitor of Candida albicans CYP51 but Fails To Bind the Human Enzyme. Antimicrobial Agents and Chemotherapy, 58(12), 7121-7127.
https://doi.org/10.1128/AAC.03707-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18693
Hull, C., Loveridge, J., Rolley, N., Donnison, I., Kelly, S., & Kelly, D. (2014). Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock. Biotechnology for Biofuels, 7(1), 133
https://doi.org/10.1186/s13068-014-0133-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18617
Hull, C., Loveridge, J., Donnison, I., Kelly, D., & Kelly, S. (2014). Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept. AMB Express, 4(1), 64
https://doi.org/10.1186/s13568-014-0064-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18763
Hawkins, N., Cools, H., Sierotzki, H., Shaw, M., Knogge, W., Kelly, S., Kelly, D., & Fraaije, B. (2014). Paralog Re-Emergence: A Novel, Historically Contingent Mechanism in the Evolution of Antimicrobial Resistance. Molecular Biology and Evolution, 31(7), 1793-1802.
https://doi.org/10.1093/molbev/msu134, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18161
http://dx.doi.org/10.1093/molbev/msu134
Fan, J., Urban, M., Parker, J., Brewer, H., Kelly, S., Hammond-Kosack, K., Fraaije, B., Liu, X., & Cools, H. (2013). Characterization of the sterol 14α-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function. New Phytologist-n/a.
https://doi.org/10.1111/NPH.12193, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14421
Nie, D., Hoobler, E., Rai, G., Warrilow, A., Perry, S., Smyrniotis, C., Jadhav, A., Simeonov, A., Parker, J., Kelly, D., Maloney, D., Kelly, S., & Holman, T. (2013). Discovery of a Novel Dual Fungal CYP51/Human 5-Lipoxygenase Inhibitor: Implications for Anti-Fungal Therapy. PLoS ONE, 8(6)
https://doi.org/10.1371/journal.pone.0065928, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15178
Eddouzi, J., Parker, J., Vale-Silva, L., Coste, A., Ischer, F., Kelly, S., Manai, M., & Sanglard, D. (2013). Molecular Mechanisms of Drug Resistance in Clinical Candida Species Isolated from Tunisian Hospitals. Antimicrobial Agents and Chemotherapy, 57(7), 3182-3193.
https://doi.org/10.1128/AAC.00555-13, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15187
Kelly, S. & Kelly, D. (2013). Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1612), 20120476-20120476.
https://doi.org/10.1098/rstb.2012.0476, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14006
Reid, E., Weynberg, K., Love, J., Isupov, M., Littlechild, J., Wilson, W., Kelly, S., Lamb, D., & Allen, M. (2013). Functional and structural characterisation of a viral cytochromeb5. FEBS Letters, 587(22), 3633-3639.
https://doi.org/10.1016/j.febslet.2013.09.035, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18159
http://dx.doi.org/10.1016/j.febslet.2013.09.035
Belič, A., Pompon, D., Monostory, K., Kelly, D., Kelly, S., & Rozman, D. (2013). An algorithm for rapid computational construction of metabolic networks: A cholesterol biosynthesis example. Computers in Biology and Medicine, 43(5), 471-480.
https://doi.org/10.1016/j.compbiomed.2013.02.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15174
http://dx.doi.org/10.1016/j.compbiomed.2013.02.017
Calabrese, E., Castellano, S., Santoriello, M., Sgherri, C., Quartacci, M., Calucci, L., Warrilow, A., Lamb, D., Kelly, S., Milite, C., Granata, I., Sbardella, G., Stefancich, G., Maresca, B., & Porta, A. (2013). Antifungal activity of azole compounds CPA18 and CPA109 against azole-susceptible and -resistant strains of Candida albicans. Journal of Antimicrobial Chemotherapy, 68(5), 1111-1119.
https://doi.org/10.1093/jac/dks506, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14005
Kelly, S., Kelly, D., Parker, J., & Warrilow, A. (2013). Azole Affinity of Sterol 14alpha-Demethylase (CYP51) Enzymes from Candida albicans and Homo sapiens. Antimicrobial Agents and Chemotherapy, 57(3)-1360.
https://doi.org/10.1128/AAC.02067-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14415
Higgins, J., Pinjon, E., Oltean, H., White, T., Kelly, S., Martel, C., Sulivan, D., Coleman, D., Moran, G., & Hull, C. (2012). Triclosan anatagonizes fluconazole activity against candida albicans. Journal of Dental Research, 91(1), 65-70.
https://doi.org/10.1177/0022034511425046, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10166
Zhao, B., Moody, S., Hider, R., Lei, L., Kelly, S., Waterman, M., & Lamb, D. (2012). Structural Analysis of Cytochrome P450 105N1 Involved in the Biosynthesis of the Zincophore, Coelibactin. International Journal of Molecular Sciences, 13(7), 8500-8513.
https://doi.org/10.3390/ijms13078500, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13027
http://dx.doi.org/10.3390/ijms13078500
Hull, C., Bader, O., Parker, J., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Two Clinical Isolates of Candida glabrata Exhibiting Reduced Sensitivity to Amphotericin B Both Harbor Mutations inERG2. Antimicrobial Agents and Chemotherapy, 56(12), 6417-6421.
https://doi.org/10.1128/aac.01145-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13028
http://dx.doi.org/10.1128/aac.01145-12
Hull, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation inERG11and Exhibiting Cross-Resistance to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 56(8), 4223-4232.
https://doi.org/10.1128/aac.06253-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12338
http://dx.doi.org/10.1128/aac.06253-11
Vale-Silva, L., Coste, A., Ischer, F., Parker, J., Kelly, S., Pinto, E., & Sanglard, D. (2012). Azole Resistance by Loss of Function of the Sterol Δ5,6-Desaturase Gene (ERG3) in Candida albicans Does Not Necessarily Decrease Virulence. Antimicrobial Agents and Chemotherapy, 56(4), 1960-1968.
https://doi.org/10.1128/aac.05720-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6836
Moody, S., Zhao, B., Lei, L., Mullins, J., Waterman, M., Kelly, S., & Lamb, D. (2012). Investigating conservation of the albaflavenone biosynthetic pathway and CYP170 bifunctionality in streptomycetes.. FEBS J., 279(9), 1640-1649.
https://doi.org/10.1111/j.1742-4658.2011.08447.x., SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10672
Agnew, C., Warrilow, A., Burton, N., Lamb, D., Kelly, S., & Brady, R. (2011). An Enlarged, Adaptable Active Site in CYP164 Family P450 Enzymes, the Sole P450 in Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 56(1), 391-402.
https://doi.org/10.1128/AAC.05227-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6364
Mullins, J., Parker, J., Cools, H., Togawa, R., Lucas, J., Fraaije, B., Kelly, D., & Kelly, S. (2011). Molecular Modelling of the Emergence of Azole Resistance in Mycosphaerella graminicola. PLoS ONE, 6(6), e20973
https://doi.org/10.1371/journal.pone.0020973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6839
Cools, H., Mullins, J., Fraaije, B., Parker, J., Kelly, D., Lucas, J., & Kelly, S. (2011). Impact of Recently Emerged Sterol 14 alpha-Demethylase (CYP51) Variants of Mycosphaerella graminicola on Azole Fungicide Sensitivity. Applied and Environmental Microbiology, 77(11), 3830-3837.
https://doi.org/10.1128/AEM.00027-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6846
Martel, C., Parker, J., Jackson, C., Warrilow, A., Rolley, N., Greig, C., Morris, S., Donnison, I., Kelly, D., Kelly, S., & Hull, C. (2011). Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol. Bioresource Technology, 102(2), 1503-1508.
https://doi.org/10.1016/j.biortech.2010.07.099, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6360
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., Kelly, S., Parker, J., & Hull, C. (2010). A Clinical Isolate of Candida albicans with Mutations in ERG11 (Encoding Sterol 14alpha-Demethylase) and ERG5 (Encoding C22 Desaturase) Is Cross Resistant to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 54(9), 3578-3583.
https://doi.org/10.1128/AAC.00303-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6353
Warrilow, A., Melo, N., Martel, C., Parker, J., Nes, W., Kelly, S., & Kelly, D. (2010). Expression, Purification, and Characterization of Aspergillus fumigatus Sterol 14alpha-Demethylase (CYP51) Isoenzymes A and B. Antimicrobial Agents and Chemotherapy, 54(10), 4225-4234.
https://doi.org/10.1128/AAC.00316-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6354
Warrilow, A., Martel, C., Parker, J., Melo, N., Lamb, D., Nes, W., Kelly, D., Kelly, S., & Hull, C. (2010). Azole Binding Properties of Candida albicans Sterol 14alpha-Demethylase (CaCYP51). Antimicrobial Agents and Chemotherapy, 54(10), 4235-4245.
https://doi.org/10.1128/AAC.00587-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6355
Martel, C., Parker, J., Warrilow, A., Rolley, N., Kelly, S., Kelly, D., Parker, J., Hull, C., & Warrilow, A. (2010). Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14alpha-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B. Antimicrobial Agents and Chemotherapy, 54(11), 4920-4923.
https://doi.org/10.1128/AAC.00349-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6356
Lamb, D., Lei, L., Zhao, B., Yuan, H., Jackson, C., Warrilow, A., Skaug, T., Dyson, P., Dawson, E., Kelly, S., Hachey, D., & Waterman, M. (2010). Streptomyces coelicolor A3(2) CYP102 Protein, a Novel Fatty Acid Hydroxylase Encoded as a Heme Domain without an N-Terminal Redox Partner. Applied and Environmental Microbiology, 76(6), 1975-1980.
https://doi.org/10.1128/AEM.03000-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6357
Martel, C., Warrilow, A., Jackson, C., Mullins, J., Togawa, R., Parker, J., Morris, M., Donnison, I., Kelly, D., Kelly, S., Mullins, J., & Hull, C. (2010). Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans. Bioresource Technology, 101(12), 4395-4402.
https://doi.org/10.1016/j.biortech.2010.01.084, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6359
Parker, J., Warrilow, A., Cools, H., Martel, C., Nes, W., Fraaije, B., Lucas, J., Kelly, D., Kelly, S., & Hull, C. (2010). Mechanism of Binding of Prothioconazole to Mycosphaerella graminicola CYP51 Differs from That of Other Azole Antifungals. Applied and Environmental Microbiology, 77(4), 1460-1465.
https://doi.org/10.1128/AEM.01332-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6361
Cheng, Q., Lamb, D., Kelly, S., Lei, L., Guenguerich, F., & Kelly, D. (2010). Cyclization of a Cellular Dipentaenone by Streptomyces coelicolor Cytochrome P450 154A1 without Oxidation/Reduction. Journal of the American Chemical Society, 132(43), 15173-15175.
https://doi.org/10.1021/ja107801v, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9901
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Rolley, N., Kelly, D., Kelly, S., & Hull, C. (2010). Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans. Antimicrobial Agents and Chemotherapy, 54(11), 4527-4533.
https://doi.org/10.1128/AAC.00348-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6358
Cools, H., Parker, J., Kelly, D., Lucas, J., Fraaije, B., & Kelly, S. (2010). Heterologous Expression of Mutated Eburicol 14α-Demethylase (CYP51) Proteins of Mycosphaerella graminicola To Assess Effects on Azole Fungicide Sensitivity and Intrinsic Protein Function. Applied and Environmental Microbiology, 76(9), 2866-2872.
https://doi.org/10.1128/aem.02158-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6856
http://dx.doi.org/10.1128/aem.02158-09
Lamb, D., Lei, L., Warrilow, A., Lepesheva, G., Mullins, J., Waterman, M., Kelly, S., & Mullins, J. (2009). The First Virally Encoded Cytochrome P450. Journal of Virology, 83(16), 8266-8269.
https://doi.org/10.1128/JVI.00289-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6352
Zhao, B., Lei, L., Vassylyev, D., Lin, X., Cane, D., Kelly, S., Yuan, H., Lamb, D., & Waterman, M. (2009). Crystal structure of albaflavenone monoxygenase containing a moonlighting terpene synthase active site.. Journal of Biological Chemistry, 284(52), 36711-9.
https://doi.org/10.1074/jbc.M109.064683, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10330
Jackson, C., Barton, R., Clark, C., & Kelly, S. (2009). Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi. Medical Mycology, 47(6), 609-617.
https://doi.org/10.1080/13693780802385445, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10333
Melo, N., Moran, G., Warrilow, A., Dudley, E., Smith, S., Sullivan, D., Lamb, D., Kelly, D., Coleman, D., & Kelly, S. (2008). CYP56 (Dit2p) in Candida albicans: Characterization and Investigation of Its Role in Growth and Antifungal Drug Susceptibility. Antimicrobial Agents and Chemotherapy, 52(10), 3718-3724.
https://doi.org/10.1128/AAC.00446-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6349
Warrilow, A., Jackson, C., Parker, J., Marczylo, T., Kelly, D., Lamb, D., & Kelly, S. (2008). Identification, Characterization, and Azole-Binding Properties of Mycobacterium smegmatis CYP164A2, a Homolog of ML2088, the Sole Cytochrome P450 Gene of Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 53(3), 1157-1164.
https://doi.org/10.1128/AAC.01237-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6351
Palabiyik, B., Karaer, S., Arda, N., Toker, S., Temizkan, G., Kelly, S., & Sarikaya, A. (2008). Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe. Biologia, 63(1), 450-454.
https://doi.org/10.2478/s11756-008-0069-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10676
Parker, J., Merkamm, M., Manning, N., Pompon, D., Kelly, S., & Kelly, D. (2008). Differential Azole Antifungal Efficacies Contrasted Using a Saccharomyces cerevisiae Strain Humanized for Sterol 14 alpha-Demethylase at the Homologous Locus. Antimicrobial Agents and Chemotherapy, 52(10), 3597-3603.
https://doi.org/10.1128/AAC.00517-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6835
Regina, G., D’Auria, F., Tafi, A., Piscitelli, F., Olla, S., Caporuscio, F., Nencioni, L., Cirilli, R., Torre, F., Melo, N., Kelly, S., Lamb, D., Artico, M., Botta, M., Palamara, A., & Silvestri, R. (2008). 1-[(3-Aryloxy-3-aryl)propyl]-1H-imidazoles, New Imidazoles with Potent Activity againstCandida albicansand Dermatophytes. Synthesis, Structure−Activity Relationship, and Molecular Modeling Studies. Journal of Medicinal Chemistry, 51(13), 3841-3855.
https://doi.org/10.1021/jm800009r, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10336
Zhao, B., Lin, X., Lei, L., Lei, L., Lamb, D., Kelly, S., Waterman, M., & Cane, D. (2008). Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 203(12), 8183-9.
https://doi.org/10.1074/jbc.M710421200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10337
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200

Supervision

Untitled (current)

PhD

Other supervisor: Prof David Lamb
Development and application of a novel mass spectrometry ionisation source for biological chemistry (awarded 2022)

PhD

Other supervisor: Dr Jonathan Mullins