Professor William Griffiths

Professor William Griffiths
Professor in Mass Spectrometry
Biomedical Sciences
Telephone: (01792) 295562
Room: Open Plan Office - 303
Third Floor
Institute of Life Science 1
Singleton Campus

The Griffiths – Wang research group is focused on the mass spectrometric analysis of lipids and proteins. Current projects involve the study of cholesterol biosynthesis and metabolism, particularly the formation of oxysterols and bile acids. New and improved methods for the identification, quantification, and characterization of bile acids, oxysterols, and other sterols and steroids, are being developed; and the involvement of these molecules in neurogenesis and immunity is being investigated. Complementary to this, proteomic techniques are being used to study biosynthetic and metabolizing enzymes in tissues and cells. This research fits within the College Biomarker & Genes, and Microbes & Immunity themes.

Publications

  1. Griffiths, W. Formation and metabolism of oxysterols and cholestenoic acids found in the mouse circulation: Lessons learnt from deuterium-enrichment experiments and the CYP46A1 transgenic mouse The Journal of Steroid Biochemistry and Molecular Biology 195 105475
  2. Griffiths, W. Lipidomics needs more standardization Nature Metabolism
  3. Griffiths, W. Elevated oxysterol levels in human and mouse livers reflect nonalcoholic steatohepatitis Journal of Lipid Research 60 7 1270 1283
  4. Griffiths, W. Metabolism of Non-Enzymatically Derived Oxysterols: Clues from sterol metabolic disorders Free Radical Biology and Medicine
  5. Griffiths, W. First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods The Journal of Steroid Biochemistry and Molecular Biology 190 115 125

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Teaching

  • PM-248 Amino acids, lipids and steroids

    The module reviews the pathways of fatty acid degradation (beta-oxidation) before describing the biochemistry of the multi-functional proteins that catalyse the formation of fatty acids. The synthesis of cholesterol via the mevalonate pathway is described along with the various metabolic control points and an understanding of dietary lipid transport. The further metabolism of cholesterol to oxysterols and their function is covered. The module provides a sound basis for understanding the metabolism of amino acids, lipids and steroids. The pathways of amino acid synthesis and degradation in humans are described along with an introduction to the urea cycle. The targeting of sterol biosynthesis in fungi by therapeutic agents is also covered.

  • PM-304 Biomolecular Research Project

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  • PMLM06 LC/MS Applications V: Metabolomics, Lipidomics and Bioactive lipids

    This module covers the basic concepts of metabolomics, lipidomics and analysis of bioactive lipids. The module will focus on the application of LC-MS analysis of biomolecules isolated from complex mixtures.

  • PMLM07 Data analysis and method development

    This module will allow students to statistically evaluate their data acquired using LC-MS to provide meaningful conclusions for their work. This will involve the practical use of `state-of-the-art¿ databases and software analysis tools available to the mass spectrometry community. Students will gain experience using the latest software technology and techniques for their future careers.

  • PMLM09 Dissertation: MS experimental project.

    Students will have the opportunity to join an established research group to work in a modern and well equipped laboratory or alternatively undertake a suitable non-laboratory based project. In this module the student will be able to gain extensive `hands on¿ specialist expertise in a chosen topic which could be targeted to their future career in the field of mass spectrometry.

Supervision

  • Development and Validation of Assays for Vitamins D and other Sterols using LC-MS and GC-TOF-MS with select -eV (current)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
  • Biomarker, cellular and genomic factors that define/characterise bacterial sepsis (current)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
    Other supervisor: Prof Thomas Humphrey
    Other supervisor: Dr Thomas Wilkinson
  • Untitled (current)

    Student name:
    PhD
    Other supervisor: Prof Michael Gravenor
    Other supervisor: Prof Yuqin Wang
  • Inhibiting IL-17 Production by Blocking Endogenous RORγt Agonists for the Treatment of Autoimmune Diseases (current)

    Student name:
    PhD
    Other supervisor: Prof Catherine Thornton
    Other supervisor: Dr Jonathan Mullins
    Other supervisor: Prof Yuqin Wang
  • Mass Spectroscopy Imaging of Human Brain (current)

    Student name:
    PhD
    Other supervisor: Dr Owain Howell
  • Oxysterols and the brain: Do oxysterols drive disease and is their concentration affected by high dose statin treatment?«br /» (current)

    Student name:
    PhD
    Other supervisor: Dr Owain Howell
  • 'Development of mass spectrometry based reference methods for the measurement of 25-hydroxyvitamins D2 and D3 and the discovery of related molecules in human serum' (awarded 2017)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang
  • 'Lipidomic analysis of sterols in Human T Helper cells' (awarded 2017)

    Student name:
    PhD
    Other supervisor: Prof Yuqin Wang