Dr James Cronin

Research Highlights

1. Vincent, E., Cronin, J., Panetti, S., Chambers, M., Holm, S., Owens, S., Francis, N., Finlay, D., Thornton, C., & Jones, N. (2019). Akt and STAT5 mediate naïve human CD4+ T-cell early metabolic response to TCR stimulation. Nature Communications, 10(1)

   https://doi.org/10.1038/s41467-019-10023-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49646

   http://dx.doi.org/10.1038/s41467-019-10023-4
2. Jones, N., Cronin, J., Dolton, G., Panetti, S., Schauenburg, A., Galloway, S., Sewell, A., Cole, D., Thornton, C., & Francis, N. (2017). Metabolic Adaptation of Human CD4+ and CD8+ T-Cells to T-Cell Receptor-Mediated Stimulation. Frontiers in Immunology, 8(1516)

   https://doi.org/10.3389/fimmu.2017.01516, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36275
3. Thornton, C., Cronin, J., Kanamarlapudi, V., & Sheldon, M. (2016). Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. Mucosal Immunology, 9(4), 1125-1136.

   https://doi.org/10.1038/mi.2015.131, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25017

   http://www.nature.com/mi/journal/vaop/ncurrent/full/mi2015131a.html
4. Cronin, J., McAdam, E., Danikas, A., Tselepis, C., Griffiths, P., Baxter, J., Thomas, L., Manson, J., & Jenkins, G. (2011). Epidermal Growth Factor Receptor (EGFR) Is Overexpressed in High-Grade Dysplasia and Adenocarcinoma of the Esophagus and May Represent a Biomarker of Histological Progression in Barrett's Esophagus (BE). The American Journal of Gastroenterology, 106(1), 46

   https://doi.org/10.1038/ajg.2010.433, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9800
5. Rawat, N., Alhamdani, A., McAdam, E., Cronin, J., Eltahir, Z., Lewis, P., Griffiths, P., Baxter, J., & Jenkins, G. (2012). Curcumin abrogates bile-induced NF-κB activity and DNA damage in vitro and suppresses NF-κB activity whilst promoting apoptosis in vivo, suggesting chemopreventative potential in Barrett’s oesophagus. Clinical and Translational Oncology, 14(4), 302-311.

   https://doi.org/10.1007/s12094-012-0799-x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12971
6. Cronin, J., Alhamdani, A., Griffiths, A., Baxter, J., Brown, T., Jenkins, G., Jenkins, G., & Cronin, J. (2011). In vitro and ex vivo models of extended reflux exposure demonstrate that weakly acidic mixed reflux heightens NF-kB-mediated gene expression. Diseases of the Esophagus, 24(5), 360-370.

   https://doi.org/10.1111/j.1442-2050.2010.01148.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12975
7. Cronin, J., Williams, L., McAdam, E., Eltahir, Z., Griffiths, P., Baxter, J., & Jenkins, G. (2010). The role of secondary bile acids in neoplastic development in the oesophagus. Biochemical Society Transactions, 38(2), 337

   https://doi.org/10.1042/BST0380337, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12976
8. Turner, M., Cronin, J., Noleto, P., Sheldon, I., & Sheldon, M. (2016). Glucose Availability and AMP-Activated Protein Kinase Link Energy Metabolism and Innate Immunity in the Bovine Endometrium. PLOS ONE, 11(3), e0151416

   https://doi.org/10.1371/journal.pone.0151416, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa26753
9. Sheldon, I., Cronin, J., Healey, G., Gabler, C., Heuwieser, W., Streyl, D., Bromfield, J., Miyamoto, A., Fergani, C., Dobson, H., Cronin, J., Healey, G., & Sheldon, M. (2014). Innate immunity and inflammation of the bovine female reproductive tract in health and disease. Reproduction, 148(3), R41-R51.

   https://doi.org/10.1530/REP-14-0163, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18387
10. Sheldon, I., Healey, G., Amos, M., Bromfield, J., Cronin, J., & Cronin, J. (2012). Future concepts in combating uterine disease depend on understanding the mechanisms of infection and immunity. , 20

    SU Repository: https://cronfa.swan.ac.uk/Record/cronfa26071

    http://www.scopus.com/inward/record.url?eid=2-s2.0-84889580282&partnerID=MN8TOARS
11. Sheldon, I., Cronin, J., Pospiech, M., Turner, M., & Sheldon, M. (2018). Mechanisms linking metabolic stress with innate immunity in the endometrium. Journal of Dairy Science, 101, 3655-3664.

    https://doi.org/10.3168/jds.2017-13135, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa39072
12. Sheldon, I., Cronin, J., Borges, A., & Cronin, J. (2011). The postpartum period and modern dairy cow fertility Part 1: Uterine function. Livestock, 16(4), 14-18.

    https://doi.org/10.1111/j.2044-3870.2011.00031.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18391
13. Sheldon, I., Cronin, J., Borges, A., & Cronin, J. (2011). The postpartum period and dairy cow fertility Part 2: Ovarian function. Livestock, 16(5), 20-24.

    https://doi.org/10.1111/j.2044-3870.2011.00052.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18392
14. Sheldon, I., Cronin, J., Goetze, L., Donofrio, G., Schuberth, H., Cronin, J., & Sheldon, M. (2009). Defining Postpartum Uterine Disease and the Mechanisms of Infection and Immunity in the Female Reproductive Tract in Cattle. Biology of Reproduction, 81(6), 1025-1032.

    https://doi.org/10.1095/biolreprod.109.077370, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12979
15. Sheldon, I., Price, S., Cronin, J., Gilbert, R., Gadsby, J., Cronin, J., & Sheldon, M. (2009). Mechanisms of Infertility Associated with Clinical and Subclinical Endometritis in High Producing Dairy Cattle. Reproduction in Domestic Animals, 44, 1-9.

    https://doi.org/10.1111/j.1439-0531.2009.01465.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12977
16. Herath, S., Lilly, S., Santos, N., Gilbert, R., Goetze, L., Bryant, C., White, J., Cronin, J., Sheldon, I., & Sheldon, M. (2009). Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reproductive Biology and Endocrinology, 7(1), 55

    https://doi.org/10.1186/1477-7827-7-55, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12978
17. Cronin, J., Mesher, D., Purdie, K., Evans, H., Breuer, J., Harwood, C., McGregor, J., Proby, C., & Cronin, J. (2008). β-Papillomaviruses and psoriasis: an intra-patient comparison of human papillomavirus carriage in skin and hair. British Journal of Dermatology, 159(1), 113-119.

    https://doi.org/10.1111/j.1365-2133.2008.08627.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa26072

    http://www.scopus.com/inward/record.url?eid=2-s2.0-45749133422&partnerID=MN8TOARS
18. Brown, V., Harwood, C., Crook, T., Cronin, J., Kelsell, D., & Proby, C. (2004). p16INK4a and p14ARF Tumor Suppressor Genes Are Commonly Inactivated in Cutaneous Squamous Cell Carcinoma. Journal of Investigative Dermatology, 122(5), 1284-1292.

    https://doi.org/10.1111/j.0022-202X.2004.22501.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18389
19. Carneiro, L., Bedford, C., Jacca, S., Rosamilia, A., Lima, V., Donofrio, G., Sheldon, I., Cronin, J., & Sheldon, M. (2017). Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus. Frontiers in Immunology, 8

    https://doi.org/10.3389/fimmu.2017.00996, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34992

    http://journal.frontiersin.org/article/10.3389/fimmu.2017.00996/full
20. Carneiro, L., Cronin, J., & Sheldon, M. (2016). Mechanisms linking bacterial infections of the bovine endometrium to disease and infertility. Reproductive Biology, 16(1), 1-7.

    https://doi.org/10.1016/j.repbio.2015.12.002, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25302
21. Preta, G., Cronin, J., Sheldon, I., & Sheldon, M. (2015). Dynasore - not just a dynamin inhibitor. Cell Communication and Signaling, 13(1)

    https://doi.org/10.1186/s12964-015-0102-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20642

    http://www.biosignaling.com/content/pdf/s12964-015-0102-1.pdf
22. Preta, G., Lotti, V., Cronin, J., Sheldon, I., Cronin, J., & Sheldon, M. (2015). Protective role of the dynamin inhibitor Dynasore against the cholesterol-dependent cytolysin of Trueperella pyogenes. The FASEB Journal, 29(4), 1516-1528.

    https://doi.org/10.1096/fj.14-265207, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19859

    http://www.fasebj.org/content/29/4/1516.full
23. Healy, L., Cronin, J., Sheldon, I., Cronin, J., & Healy, L. (2015). Polarized Epithelial Cells Secrete Interleukin 6 Apically in the Bovine Endometrium. Biology of Reproduction, 92(6), 151-151.

    https://doi.org/10.1095/biolreprod.115.127936, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa24091
24. Cronin, J., Hodges, R., Pedersen, S., Sheldon, I., & Sheldon, M. (2014). Enzyme Linked Immunosorbent Assay for Quantification of Bovine Interleukin-8 to Study Infection and Immunity in the Female Genital Tract. American Journal of Reproductive Immunology, n/a-n/a.

    https://doi.org/10.1111/aji.12344, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19649
25. Healy, L., Cronin, J., Sheldon, I., & Sheldon, M. (2014). Endometrial cells sense and react to tissue damage during infection of the bovine endometrium via interleukin 1. Scientific Reports, 4, 7060

    https://doi.org/10.1038/srep07060, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19258
26. Turner, M., Cronin, J., Healey, G., Sheldon, I., & Sheldon, M. (2014). Epithelial and Stromal Cells of Bovine Endometrium Have Roles in Innate Immunity and Initiate Inflammatory Responses to Bacterial Lipopeptides In Vitro via Toll-Like Receptors TLR2, TLR1, and TLR6. Endocrinology, 155(4), 1453-1465.

    https://doi.org/10.1210/en.2013-1822, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa17657

    http://press.endocrine.org/doi/pdf/10.1210/en.2013-1822
27. Cronin, J., Turner, M., Goetze, L., Bryant, C., Sheldon, I., Cronin, J., Sheldon, M., & Turner, M. (2012). Toll-Like Receptor 4 and MYD88-Dependent Signaling Mechanisms of the Innate Immune System Are Essential for the Response to Lipopolysaccharide by Epithelial and Stromal Cells of the Bovine Endometrium. Biology of Reproduction, 86(2), 51-51.

    https://doi.org/10.1095/biolreprod.111.092718, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa11551
28. Price, J., Cronin, J., Sheldon, I., & Sheldon, M. (2012). Toll-Like Receptor Expression and Function in the COV434 Granulosa Cell Line. American Journal of Reproductive Immunology, n/a-n/a.

    https://doi.org/10.1111/j.1600-0897.2011.01103.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa11550
29. Williams, L., Somasekar, A., Davies, D., Cronin, J., Doak, S., Alcolado, R., Williams, J., Griffiths, A., Baxter, J., Jenkins, G., Jenkins, G., Cronin, J., Williams, J., & Baxter, J. (2009). Aneuploidy involving chromosome 1 may be an early predictive marker of intestinal type gastric cancer. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 669(1-2), 104

    https://doi.org/10.1016/j.mrfmmm.2009.05.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9801
30. Baxter, J., Jenkins, G., & Cronin, J. (2008). The bile acid deoxycholic acid has a non-linear dose response for DNA damage and possibly NF-kappaB activation in oesophageal cells, with a mechanism of action involving ROS. , 23(5), 399-405.

    https://doi.org/10.1093/mutage/gen029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9803
31. Jenkins, G. & Cronin, J. (2008). Chapter 6. Bile Acids and Oesophageal Adenocarcinoma (OA). Bile Acids-121).

    https://doi.org/10.1039/9781847558336-00100, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa23428

    http://www.scopus.com/inward/record.url?eid=2-s2.0-84897485710&partnerID=MN8TOARS
32. Jones, N., Vincent, E., Felix, L., Cronin, J., Scott, L., Hole, P., Lacy, P., & Thornton, C. (2020). Interleukin‐5 drives glycolysis and reactive oxygen species‐dependent citric acid cycling by eosinophils. Allergy, 75(6), 1361-1370.

    https://doi.org/10.1111/all.14158, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa53068

    http://dx.doi.org/10.1111/all.14158

All Research

• Repurposed drug targeting of metabolic flexibility in the tumour microenvironment (current)

  PhD

  Other supervisor: Dr Nicholas Jones

• Application of Benchmark Dose analysis to in vitro genotoxicity data for compound risk characterisation (current)

  PhD

  Other supervisor: Dr George Johnson

• Investigating the potential for induction of ferroptotic programmed cell death by statins in chemotherapy-resistant ovarian cancer (current)

  PhD

  Other supervisor: Dr Matthew Hitchings

• Maternal Immunometabolism Adaptation to Pregnancy (current)

  PhD

  Other supervisor: Prof Catherine Thornton

• Cellular regulation of protection against cholesterol-dependent cytolysins by steroids and oxysterols (current)

  PhD

  Other supervisor: Prof Martin Sheldon

• Targeting iron metabolism in cancer to induce ferroptosis: a type of programmed cell death (current)

  PhD

  Other supervisor: Prof Martin Sheldon

• Regulation of Metabolism in Multiple Myeloma (MM) in Humans (current)

  PhD

  Other supervisor: Prof Catherine Thornton

• The development and application of a MeDIP assay to determine a link between epigenetics and metabolism in ovarian cancer progression (current)

  PhD

  Other supervisor: Dr Lewis Francis

• Role of Mitochondrial pyruvate carrier in ovarian cancer progression (current)

  PhD

  Other supervisor: Prof Deyarina Gonzalez

  Other supervisor: Prof Martin Sheldon

• Multiplexed in vitro assay for genetic toxicology screening (current)

  PhD

  Other supervisor: Prof Paul Rees

  Other supervisor: Dr George Johnson

• Bisphosphonate inhibitors of squalene synthase protect human tissue cells against cholesterol dependent cytolysins from pathogenic bacteria (awarded 2020)

  PhD

  Other supervisor: Prof Martin Sheldon

• Candidate gene vs genome-wide association study approaches in ASD: A systematic review to determine any overlapping genes. (current)

  MRes

  Other supervisor: Dr Owen Bodger

• The role of lipoxygenases in evasion of ferroptotic programmed cell death in ovarian cancer (current)

  MSc

  Other supervisor: Dr Nicholas Jones

• Applying a ground truth based neural network approach to automating the micronucleus assay, using nitrosamines as a case study (current)

  MSc

  Other supervisor: Dr George Johnson

• Analysis and Toxicological Assay Development Following the Assessment of Micronuclei Induction and Immune Response Pathways in Chemo-Sensitive and Chemo-Resistant Ovarian Cancer Models (awarded 2020)

  MSc

  Other supervisor: Dr George Johnson

• Targeting Therapy-Resistant Ovarian Cancer by Induction of Ferroptotic Cell Death (awarded 2019)

  MSc

  Other supervisor: Prof Martin Sheldon

• PM-249 Human Immunology

  The course is designed as an introduction to immunology and the human immune system. The course covers thefundamentals of immunology including functional perturbations associated with disease and experimentalapproaches to the study of immunology.

• PM-280 Skills for Researchers

  The modern scientist graduate is expected to have gained extensive laboratory-based experience and this is certainly essential for any student intent on pursuing a research-based career. Skills for Researchers provides students with the opportunity to acquire the core skills to be able to conduct, analyse, interpret and present scientific experiments. A large proportion of the module will be spent in the laboratory gaining hands-on experience. Skills acquired in this module will form the basis for future experimental work, including the final year project. Students will have the opportunity to provide feedback to peers and engage with a variety of online learning materials.

• PM-300 Medical Genetics

  The course is designed to provide an advanced study of the identification of human genes and the determination of the influence of human genes upon disease and health status. Gene identification provides targets for the development of new pharmaceuticals and the range of variation present in the population.

• PM-347 Human Immunopathology

  The module aims to provide students with a greater understanding of the human immune system and the causes of a range of diseases associated with immune dysfunction including autoimmune diseases, metabolic disorders and neurological conditions.

• PM-357 Biomedical Laboratory Techniques

  The module will provide practical and in depth theory of applications and equipment available to MSci students in the biomedical research laboratories based at the Medical School. The module will provide guidelines and rationale for experimental design, and data and statistical analysis.

• PM-357C Technegau Labordy Biofeddygol

  Bydd y modiwl yn darparu damcaniaeth ymarferol a manwl o¿r cymwysiadau ac offer sydd ar gael i fyfyrwyr MSci yn y labordai ymchwil biofeddygol sydd wedi'u lleoli yn yr Ysgol Feddygaeth. Bydd y modiwl yn darparu canllawiau a rhesymeg ar gyfer cynlluniau arbrofol, a dadansoddiad data ac ystadegau.

• PM-400 Advanced Research Project A

  The advanced research project is a key component of the final year of study, providing students with experience of conducting cutting-edge research in the Institute of Life Science and Centre for Nanohealth over an extended period of time. The project will fall into one of the current medically-related research themes: Biomarkers and Genes; Microbes and Immunity; Devices and health informatics. Students will employ a range of advanced analytical procedures to investigate a specific topic. In addition, they will gain experience in preparing a research proposal and presenting their data in various formats. Research topics will be assigned that are appropriate to a specific degree title. For example, a Genetics student could be assigned a project investigating gene function in an insect vector of a tropical disease, using the technique of RNA interference. The advanced research project is divided between 2 modules, PM-400 and PM-402. PM-400 includes the following components: (1) Preparation of a research poster, (2) A 15-minute audio recording giving a presentation of the research area and (3) Oral defence of the project in a viva.

• PM-402 Advanced Research Project B

  The advanced research project is a key component of the final year of study, providing students with experience of conducting cutting-edge research in the Institute of Life Science and Centre for Nanohealth over an extended period. The project will fall into one of the current medically-related research themes: Biomarkers and Genes; Microbes and Immunity; Devices. Students will employ a range of advanced analytical procedures to investigate a specific topic. In addition, they will gain experience in preparing a research proposal and presenting their data in various formats. Research topics will be assigned that are appropriate to a specific degree title. For example, a Genetics student could be assigned a project investigating gene function in an insect vector of a tropical disease, using the technique of RNA interference. The advanced research project is divided between 2 modules, PM-400 and PM-402. PM-402 comprises the final written dissertation.

• PMNM11 Nanomedicines, pharmaceuticals and advanced therapeutics

  This module will explore the history and development of molecular medicines and pharmaceuticals, providing the basis for an advanced understanding of next generation therapeutic approaches. Using landmark technology and chemical development phases informed by separation science and mass spectrometry, the module uses an application driven approach to provide the student with an extensive knowledgebase of drug development, the pharmaceutical industry and nanotherapeutics. Students will be able to characterize and map the path of a drug from administration, to metabolism and elimination, and critically evaluate drug design and delivery approaches. Traditional small chemical entities used in molecular medicine will be outlined, using drugs such as taxols and tamoxifen as exemplars. Common target oncology and non-oncology disorders will provide the context; with students encouraged to explore targeted nanoparticle fabrication, drug encapsulation and release profiling, from early first generation drugs such as Abraxane to second generation biologically targeted SMART delivery systems. Future molecular medicines such as antibody drug conjugates and kinase inhibitors will be taught by guest lectures from industry and clinicians, covering the spectrum of drug development to delivery and clinical considerations.

• PMZM15 Nanomedicines and Therapeutics

  The module will explore the history and development of molecular medicines. Using landmark technology and chemical development phases, the traditional small chemical entities using in molecular medicine will be outlined, using drugs such as taxols and tamoxifen as exemplars. The common target oncology and non oncology disorders will outlined and their respective targets for such medicines detailed alongside drug modes of action and delivery (IV and oral). Further exploration of targeted nanoparticle delivery, from early first generation drugs such as Abraxane to second generation biologically targeted SMART delivery systems, will expand the knowledge to future molecular medicines such as antibody drug conjugates and kinase inhibitors. Excitingly the module will include guest lectures from industry and clinicians, covering the spectrum of drug development to delivery and clinical considerations.

• Senior Lecturer, Swansea University Medical School

  2017 - Present

• Co-Director Post-Graduate Research, Swansea University Medical School

  2017 - Present