Professor Shareen Doak
Personal Chair
Biomedical Sciences
Telephone: (01792) 295388
Room: Academic Office - 404
Fourth Floor
Institute of Life Science 1
Singleton Campus

Shareen Doak is Professor of Genotoxicology and Cancer in Swansea University Medical School. Shareen is the Medical School's Director of the Centre for NanoHealth and she leads the In Vitro Toxicology Group. Shareen is a UK and EUROTOX Registered Toxicologist, an invited Fellow of the Royal Society of Biology (FRSB) and an elected Fellow of the Learned Society of Wales (FLSW).

Shareen sits on the UK Government Committee on Mutagenicity (COM), and is the Nanomaterials Working Group Co-Leader for the International Genetic Toxicology Technical Committee (GTTC). She is also Senior Editor for Mutagenesis.

Shareen Coordinates the €13Million H2020 PATROLS project (www.patrols-h2020.eu) and is Director of the €12Million  Celtic Advanced Life Science Innovation Network (CALIN), an Ireland-Wales INTERREG operation established to build a innovation bridge between Wales and Ireland in life sciences.

Shareen’s research interests focus on the genotoxic profiles of engineered nanomaterials, the mechanisms underlying their DNA damaging potential and subsequent consequences upon human health. Her interests extend to the development of advanced 3D culture models and mechanism-based bioassays for safety assessment to reduce the need for animal testing.  While her prostate cancer research focuses on understanding the molecular basis of progression to invasive, aggressive disease; with an ultimate aim of identifying a prognostic biomarker panel for improved clinical management of patients.

Areas of Expertise

  • Genetic Toxicology
  • Nanotoxicology
  • DNA Damage Mechanisms
  • Biomarkers / Molecular Biology of Prostate Cancer

Publications

  1. Dunstan, P, Doak, S, Walker, K Mechanisms of cell–cell adhesion identified by immunofluorescent labelling with quantum dots: A scanning near-field optical microscopy approachUltramicroscopy111812001205
  2. Parry, J, Baxter, J, Shah, V, Griffiths, A, Parry, E, Jenkins, G, Doak, S Characterisation of p53 status at the gene, chromosomal and protein levels in oesophageal adenocarcinomaBritish Journal of Cancer89917291735
  3. Doak, S Chromosome 4 hyperploidy represents an early genetic aberration in premalignant Barrett's oesophagusGut525623628
  4. BAYLIS, R, DOAK, S, PARRY, J, DUNSTAN, P Chromosome morphology after long-term storage investigated by scanning near-field optical microscopyJournal of Microscopy2213177182
  5. Dunstan, P, Doak, S, Morgan, C, Walker, K, Schnur, J Quantum Dots for Multiplexed Detection and Characterisation of Prostate Cancer Cells Using a Scanning Near-Field Optical MicroscopePLoS ONE72e31592
  6. Jenkins, G, Williams, P, Wright, C, Barron, A, Brown, A, Hondow, N, Dunstan, P, Rickman, R, Brady, K, Doak, S, Manshian, B Single-walled carbon nanotubes: differential genotoxic potential associated with physico-chemical propertiesNanotoxicology72144156
  7. Johnson, G, Jenkins, G, Doak, S, Thomas, A Vinblastine and diethylstilboestrol tested in the in vitro mammalian cell micronucleus test (MNvit) at Swansea University UK in support of OECD draft Test Guideline 487Mutation Research/Genetic Toxicology and Environmental Mutagenesis7022189192
  8. Jenkins, G, Zaïr, Z, Doak, S, Johnson, G Genotoxic thresholds, DNA repair, and susceptibility in human populationsToxicology2783305310
  9. Johnson, G, Brown, K, Zair, Z, Jenkins, G, Doak, S, Singh, R N-Methylpurine DNA Glycosylase Plays a Pivotal Role in the Threshold Response of Ethyl Methanesulfonate-Induced Chromosome DamageToxicological Sciences1192346358
  10. Seager, A, Shah, U, Mikhail, J, Nelson, B, Marquis, B, Doak, S, Johnson, G, Griffiths, S, Carmichael, P, Scott, S, Jenkins, G, Scott, A Pro-oxidant Induced DNA Damage in Human Lymphoblastoid Cells: Homeostatic Mechanisms of Genotoxic ToleranceToxicological Sciences1282387397

See more...

Teaching

  • PM-304 Biomolecular Research Project

    *

  • PM-M28 Nano(geno)toxicology

    The field of nanotechnology is ever increasing in size, and the number of nano-sized (1-100nm) particles being produced on a daily basis is at the tonnage level. Although nanotechnology-based applications are considered to be advantageous for a plethora of human activities, the potential risks posed by human exposure to nanoparticles is a constantly developing field of research. The purpose of this module therefore is to introduce to the student to particle toxicology and how it can impact upon human health. Further to this, the students will undertake a specific practical component to complement that of the theoretical content of the module. To this extent, the students will undertake a week-long, 'mini-project', covering the major areas of nanoparticle toxicology. From this approach, it is intended that the student will experience the key points of the field.

Supervision

  • "Design and Evaluation of anti-STEAP2 antibodies to treat aggressive Prostate Cancer" (current)

    Student name:
    PhD
    Other supervisor: Prof Steve Conlan
  • Untitled (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Blood-based test for pancreatic cancer (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Development of a blood-based biomarker for early detection of oesophageal adenocarcinoma (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Mechanistic evaluation of the impact of superparamagnetic iron oxide nanoparticles conjugated with drugs (SPIONd) on intracellular signalling/homeostatic mechanisms (current)

    Student name:
    PhD
    Other supervisor: Dr Martin Clift
  • Studying the mechanistic process associated with nanoparticle genotoxicity. (current)

    Student name:
    MSc
    Other supervisor: Dr Martin Clift
  • Examining the Interaction between Adipose Derived Stem Cells (ADSC) and MCF-7 breast cancer in the breast microenvironment: a pilot study (current)

    Student name:
    MPhil
    Other supervisor: Prof Iain Whitaker
  • Blood based chemotherapy response monitoring (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Patient reported outcome measures for facial skin cancer and its post-treatment reconstruction (current)

    Student name:
    PhD
    Other supervisor: Prof Iain Whitaker
    Other supervisor: Prof Hayley Hutchings
  • A Mechanistic Investigation of the Relationship between Extramural Vascular Invasion (EMVI) and CpG Island Methylation Phenotype (CIMP) in Rectal Cancer (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Developing an in vitro repeat-dose approach to detect non-genotoxic carcinogens. (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Understanding the human health impacts of graphene-based nanomaterials under occupational exposure scenarios (current)

    Student name:
    PhD
    Other supervisor: Dr Martin Clift
  • OPTIMISATION OF A NOVEL, COMPOSITE, NATURAL BIOINK FOR CARTILAGE TISSUE ENGINEERING (current)

    Student name:
    PhD
    Other supervisor: Prof Iain Whitaker
  • Characterising The Role Of Tumourigenic Markers In Prostate Cancer Invasion Through 3D Tumour-Bone Stromal Cell Models (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • An advanced multi-cellular and dynamic flow model of the human alveolar airway to study the impact of inhaled particulate (current)

    Student name:
    PhD
    Other supervisor: Dr Martin Clift
  • Investigating the human health implications of e-cigarettes: An in vitro mechanistic toxicology approach. (current)

    Student name:
    PhD
    Other supervisor: Dr Martin Clift
  • Development of a novel in vitro liver 3D model to assess potential (geno)toxicity of nanomaterials (current)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • Deducing the impacts and associated mechanisms of engineered nanoparticles upon telomere status in lung epithelial cells in vitro. (awarded 2019)

    Student name:
    MSc
    Other supervisor: Dr Martin Clift
  • Sodium Salicylate Effects On Prostate Cancer Aggressiveness And Development Of Prostate Cancer Spheroids For Drug Evaluation (awarded 2019)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • In Vitro Lung Deposition Models to Assess the Mechanistic Genotoxicity of Characterised Few-Layer Graphene (awarded 2019)

    Student name:
    PhD
    Other supervisor: Dr Martin Clift
  • Optimisation and Application of Passive Dosing Exposures in In Vitro Genotoxicity Tests (awarded 2018)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • 'In vitro assessment of genotoxicity and cell cycle abnormalities to better understand carcinogenic risk' (awarded 2018)

    Student name:
    PhD
    Other supervisor: Prof Gareth Jenkins
  • 'The molecular mechanisms for chemo-preventative effects of aspirin on prostate cancer' (awarded 2017)

    Student name:
    MSc
    Other supervisor: Prof Steve Conlan
  • 'The role of STEAP2 in Prostate Cancer Progression' (awarded 2017)

    Student name:
    PhD
    Other supervisor: Dr Claire Morgan

External Responsibilities

Research Groups

  • In Vitro Toxicology Group

    Our group focuses on the mechanistic basis for DNA damage induction and persistence, as well as its consequences to human health. The approaches taken by this group involve models of human mutagenesis in vitro in human cells, including genetically modified repair deficient cells, to understand basic mechanisms. Recent work has also focused on assessing 3D human liver and skin models for measuring DNA damage, coupled to automated micronucleus capabilities.

  • Nano(geno)toxicology

    Our research interests lie in understanding the mechanistic basis for DNA damage (genotoxicity) induction and persistence, as well as its consequences to human health following exposure to engineered nanomaterials. We focus on determining genotoxic potential as a function of nanomaterial physico-chemical characteristics and on developing advanced in vitro test systems suited to the assessment of these novel substances.

  • Prostate Cancer

    Our prostate cancer research activity focuses on the identification and validation of molecular biomarkers for the prediction of prostate cancer outcome and evaluating their use as drug targets. The ultimate aim is to achieve patient stratification according to risk for developing metastatic disease with such biomarkers, in order to improve the clinical management of patients.

Administrative Responsibilities

  • Director of Center for NanoHealth (CNH)

    2015 - Present

  • Member - College of Medicine Research Committee

    2012 - Present