Professor William Griffiths

Professor William Griffiths
Professor in Mass Spectrometry
Swansea University Medical School
Telephone: (01792) 295562

About Me

The Griffiths – Wang research group is focused on the mass spectrometric analysis of lipids and proteins. Current projects involve the study of cholesterol biosynthesis and metabolism, particularly the formation of oxysterols and bile acids. New and improved methods for the identification, quantification, and characterization of bile acids, oxysterols, and other sterols and steroids, are being developed; and the involvement of these molecules in neurogenesis and immunity is being investigated. Complementary to this, proteomic techniques are being used to study biosynthetic and metabolizing enzymes in tissues and cells. This research fits within the College Biomarker & Genes, and Microbes & Immunity themes.

Publications

  1. & Reduced Plasma Levels of 25-Hydroxycholesterol and Increased Cerebrospinal Fluid Levels of Bile Acid Precursors in Multiple Sclerosis Patients. Molecular Neurobiology
  2. & Defective cholesterol metabolism in amyotrophic lateral sclerosis. Journal of Lipid Research, jlr.P071639
  3. & The role of microRNA-155/LXR pathway in experimental and Idiopathic Pulmonary Fibrosis. Journal of Allergy and Clinical Immunology
  4. et. al. An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. PLOS Biology 14(3), e1002364
  5. & Sterols and oxysterols in plasma from Smith-Lemli-Opitz syndrome patients. The Journal of Steroid Biochemistry and Molecular Biology

See more...

Teaching

  • PMLM01 Separation science and sample handling

    This module covers the basic concepts of separation science, from analyte extraction through chromatographic separation, sample handling and ultimately to mass spectrometry-based detection. The module will focus on the application of separation science for the analysis of biomolecules isolated from complex mixtures.

  • PMLM06 LC/MS Applications V: Metabolomics, Lipidomics and Bioactive lipids

    This module covers the basic concepts of metabolomics, lipidomics and analysis of bioactive lipids. The module will focus on the application of LC-MS analysis of biomolecules isolated from complex mixtures.

  • PMLM07 Data analysis and method development

    This module will allow students to statistically evaluate their data acquired using LC-MS to provide meaningful conclusions for their work. This will involve the practical use of ‘state-of-the-art’ databases and software analysis tools available to the mass spectrometry community. Students will gain experience using the latest software technology and techniques for their future careers.

  • PMLM09 Dissertation: MS experimental project.

    Students will have the opportunity to join an established research group to work in a modern and well equipped laboratory or alternatively undertake a suitable non-laboratory based project. In this module the student will be able to gain extensive ‘hands on’ specialist expertise in a chosen topic which could be targeted to their future career in the field of mass spectrometry.

Supervision

  • Untitled (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • Lipidomic analysis of sterols in Human T Helper cells (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • Development of mass spectrometry based reference methods for the measurement of 25-hydroxyvitamins D2 and D3 and the discovery of related molecules in human serum (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • Sensing bacterial infection: 25-hydroxycholesterol as a biomarker (current)

    Student name:
    PhD
    Other supervisor: Dr Thomas Wilkinson
    Other supervisor: Dr Yuqin Wang
    Other supervisor: Prof Thomas Humphrey
  • 'Tandem Mass Tags for Metabolomics' (awarded 2016)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • 'Sterols and oxysterols in the brain and in the immune system' (awarded 2014)

    Student name:
    PhD
    Other supervisor: Prof Mark Rees
  • '''chemical proteomics to characterise the emerging roles of oxysterols and oxysterol-mediated protein-protein interactions''' (awarded 2013)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang