Professor William Griffiths

Professor William Griffiths
Professor in Mass Spectrometry
Swansea University Medical School
Telephone: (01792) 295562

The Griffiths – Wang research group is focused on the mass spectrometric analysis of lipids and proteins. Current projects involve the study of cholesterol biosynthesis and metabolism, particularly the formation of oxysterols and bile acids. New and improved methods for the identification, quantification, and characterization of bile acids, oxysterols, and other sterols and steroids, are being developed; and the involvement of these molecules in neurogenesis and immunity is being investigated. Complementary to this, proteomic techniques are being used to study biosynthetic and metabolizing enzymes in tissues and cells. This research fits within the College Biomarker & Genes, and Microbes & Immunity themes.

Publications

  1. Introduction and Overview of Lipidomic Strategies. In Neuromethods: Lipidomics.
  2. & Charge-tagging liquid chromatography–mass spectrometry methodology targeting oxysterol diastereoisomers. Chemistry and Physics of Lipids
  3. & Sterolomics: State of the Art, Developments, Limitations and Challenges. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
  4. & Cholesterolomics: An update. Analytical Biochemistry
  5. & Defective cholesterol metabolism in amyotrophic lateral sclerosis. Journal of Lipid Research, jlr.P071639

See more...

Teaching

  • PM-304 Biomolecular Research Project

    *

  • PMLM01 Separation science and sample handling

    This module covers the basic concepts of separation science, from analyte extraction through chromatographic separation, sample handling and ultimately to mass spectrometry-based detection. The module will focus on the application of separation science for the analysis of biomolecules isolated from complex mixtures.

  • PMLM06 LC/MS Applications V: Metabolomics, Lipidomics and Bioactive lipids

    This module covers the basic concepts of metabolomics, lipidomics and analysis of bioactive lipids. The module will focus on the application of LC-MS analysis of biomolecules isolated from complex mixtures.

  • PMLM07 Data analysis and method development

    This module will allow students to statistically evaluate their data acquired using LC-MS to provide meaningful conclusions for their work. This will involve the practical use of `state-of-the-art¿ databases and software analysis tools available to the mass spectrometry community. Students will gain experience using the latest software technology and techniques for their future careers.

  • PMLM09 Dissertation: MS experimental project.

    Students will have the opportunity to join an established research group to work in a modern and well equipped laboratory or alternatively undertake a suitable non-laboratory based project. In this module the student will be able to gain extensive `hands on¿ specialist expertise in a chosen topic which could be targeted to their future career in the field of mass spectrometry.

Supervision

  • 'Development of mass spectrometry based reference methods for the measurement of 25-hydroxyvitamins D2 and D3 and the discovery of related molecules in human serum' (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • 'Lipidomic analysis of sterols in Human T Helper cells' (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • Sensing bacterial infection: 25-hydroxycholesterol as a biomarker (current)

    Student name:
    PhD
    Other supervisor: Dr Thomas Wilkinson
    Other supervisor: Prof Thomas Humphrey
    Other supervisor: Dr Yuqin Wang
  • Development and Validation of Assays for Vitamins D and other Sterols using LC-MS and GC-TOF-MS with select -eV (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
  • Untitled (current)

    Student name:
    PhD
    Other supervisor: Dr Yuqin Wang
    Other supervisor: Dr Jonathan Mullins
    Other supervisor: Prof Catherine Thornton