Module Synopses

PMLM00: Mass spectrometry - basics and fundamentals

This module will enable students to gain a sound knowledgebase of the basic concepts of mass spectrometry including the individual components, linking to modern separation techniques, instrument maintenance, spectral interpretation, and the basis of modern applications such as, compound identification by generating a characteristic mass ‘fingerprint’ for the chemical structure and compound quantitation.

PMLM01: Separation science and sample handling

This module covers basic and advanced concepts of separation science, how it is integrated with several forms of compound detection, gain ‘hands-on’ problem solving experience and strategic method development for complete bioanalysis according to compound chemical characteristics and method application.  

PMLM02: LC/MS Applications I: Proteomics

This applications module will enable students to gain a sound knowledgebase of the concepts of mass spectrometry based proteomics including sample preparation and separation (protein extraction from cell and tissue, protein digestion, peptide separation), mass spectrometry analysis (peptide fragmentation and interpretation of CID spectra of peptides), protein identification by database searching, quantitative proteomics, and sample preparation and analysis of post-translational modifications (PTMs).

PMLM03: LC/MS Applications II: Pharmaceutical

Students will have the opportunity to achieve an extensive knowledgebase of drug development and the role of mass spectrometry within the pharmaceutical industry.  Students will be able to characterize and map the path of a drug from administration, to metabolism and elimination, and critically evaluate drug design.    

PMLM04: LC/MS Applications III: Environmental and Forensic Analysis

This is an applications driven module designed for students to gain a sound knowledgebase of environmental and forensic analysis by mass spectrometry and several transferable skills suitable for continued professional development.  Students will gain an insight into the role mass spectrometry has to play in agriculture and archaeology, in addition to more topical subjects such as monitoring climate change and the fight against crime and terrorism.        

PMLM05: LC/MS Applications IV: Medical and life sciences
Students can achieve an extensive knowledge of the role mass spectrometry plays in biomarker discovery (proteins and small molecules) with its application to clinical diagnosis including the analysis of carbohydrates, glycoproteins and nucleic acids using activity-based proteomics and techniques for affinity pull-down.

PMLM06: LC/MS Applications V: Metabolomics, Lipidomics and Bioactive lipids

This module covers conventional sample handling, extraction, separation techniques (including both liquid and gas chromatography) and analysis used in modern metabolomics, and lipidomics by mass spectrometry.  Students will gain a sound knowledgebase in many of facets used in these fields such as hyphenated techniques (GC-MS, LC-MS, LC-MS/MS, HR-MS, HR-MS/MS), the interpretation of data (elution profile, retention time, nominal mass, exact mass, fragmentation, MSn, library matching, databases) and quantitation.

PMLM07: Data analysis and method development

This module will allow students to statistically evaluate their data acquired using LC-MS to provide meaningful conclusions for their work.  This will involve the practical use of ‘state-of-the-art’ databases and software analysis tools available to the mass spectrometry community.  Students will gain experience using the latest software technology and techniques for their future careers.

PMLM08: Professional management and laboratory practice

Students will cover Good Laboratory, Clinical and Manufacturing Practice (GLP, GCP, GMP) including, experimental design required for assay validation approved by the Food and Drug Administration (FDA). The need and implementation of SOPs with particular focus on the pharmaceutical and medical industries, and how these correlate with stringent quality control procedures and health and safety existing due to current legislation.

PMLM09: Dissertation: MS Experimental project

Students will have the opportunity to join an established research group to work in a modern and well equipped laboratory or alternatively undertake a suitable non-laboratory based project.  In this module the student will be able to gain extensive ‘hands-on’ specialist expertise in a chosen topic which could be targeted to their future career in the field of mass spectrometry.