Polymerase Chain Reaction (PCR) System from Biorad

PCR System from Biorad

pcr

  • Real-time PCR suite that delivers sensitive, reliable detection including absolute quantification, genetic variation analysis, and gene expression
  • Automated analysis of comprehensive gene expression studies
  • Sensitive target detection for multiplex assay in a variety of real-time PCR
  • Reliable performance and higher throughput options for quantitative expression analysis

Applications

  • Gene expression (qPCR)
  • SNP genotyping
  • Copy number analysis
  • Chromatin immunoprecipitation
  • DNA methylation analysis
  • Disease diagnosis

Case Study

Comparing WT1 gene expression levels between PCOS patients and normal controls upon in-vitro decidualization.

RNA is extracted from primary stromal cells after treatment with cAMP. Primers specific for WT1 are used for PCR and the difference between Cq values shows the difference in WT1 gene expression between the two groups highlighting the importance of molecular changes that can lead to infertility.