Dr Mark Holton

Engineering Research Officer

Contact Me

Telephone: 01792 60 (2992)

Email: m.d.holton@swansea.ac.uk

About Me

With a heavy background in electronics and programming, this researcher has extensive knowledge and experience of nano- and micro-scale imaging systems looking at both hard and soft materials such as silicon lasers and biological cells.  As part of a group headed by Professor Huw Summers within the college of Engineering, he has carried numerous experiments looking at the mechanisms and mathematical description of the loading of nanoparticles into biological cells and the subsequent dispersion amongst progeny using both standard, and new, novel flow cytometry imaging systems (ImageStream).  Past work includes looking at the fluorescent lifetime of nanoparticles in biological systems using pulsed white-laser systems, and scanning near-field optical microscopy of quantum dots and vertical cavity surface emitting lasers.  At one time part of the Swansea-Nano Knowledge Transfer Centre in semiconductor & photonics, he developed multiple electronic systems (both circuitry and firmware) including controlled LED devices for enhanced growth of E-Coli, processor controlled high frequency pulsed magnetic field generators for the study of improving the rates of wound healing, and most recently, discrete sensor logging devices for attachment to small insects/bugs, wild animals and marine fish/mammals to capture high frequency movement and environmental data.  His latest work has involved the further development of the animal data loggers for further use in Sports Science for monitoring injury and recovery mechanisms, and working with a group based at the Physics and Astronomy group in Cardiff University, developing miniaturised biological cell counters using sub-millimetre sized lasing devices for handheld analysers. 

Areas of Expertise

Electronics analogue/digital design, maintenance, and repair

Programming (MatLab, C++, Python, 6502, 68000)

Nanoscale topographic and optical imaging of hard and biological materials

Imaging and spectroscopy of nanoparticles

Flow cytometry and novel adapted systems

Development of high frequency data logging systems for the study of animals and insects

Publications

Mitigating the squash effect: sloths breathe easily upside down, Rebecca N. Cliffe, Judy A. Avey-Arroyo, Francisco J. Arroyo, Mark D. Holton, Rory P. Wilson, Biology Letters, 10(4), 20141072

Quantification of Nanoparticle Dose and Vesicular Inheritance in Proliferating Cells, Huw D Summers, Martyn R Brown, Mark D Holton, James A Tonkin, Nicole Hondow, Andrew P Brown, Rik Brydson, Paul Rees, ACS Nano 06/2013

TEM analysis of nanoparticle dispersions with application towards the quantification of in vitro cellular uptake, N Hondow, P Wang, R Brydson, M D Holton, P Rees, H D Summers, A Brown, Journal of Physics Conference Series 37(1) 01/2012

Quantitative characterization of nanoparticle agglomeration within biological media, Nicole Hondow, Rik Brydson, Peiyi Wang, Mark D. Holton, M. Rowan Brown, Paul Rees, Huw D. Summers, Andy Brown, Journal of Nanoparticle Research, 14(7), 01/2012

A transfer function approach to measuring cell inheritance, Paul Rees, M Rowan Brown, Huw D Summers, Mark D Holton, Rachel J Errington, Sally C Chappell, Paul J Smith, BMC Systems Biology, 5:31, 02/2011

Statistical analysis of nanoparticle dosing in a dynamic cellular system, Huw D Summers, Mark D Holton, Paul Rees, Paul M Williams, Catherine A Thornton, Cytometry A, 77(10), 933-9, 10/2010

Stroboscopic fluorescence lifetime imaging, Mark D Holton, Oscar R Silvestre, Rachel J Errington, Paul J Smith, Daniel R Matthews, Paul Rees, Huw D Summers, Optics Express, 5202-15, 17(7), 04/2009

Fluorescence lifetime based contrast imaging using variable period excitation pulse trains, M. D. Holton, O. F. Silvestre, R. J. Errington, P. J. Smith, P. Rees, H. D. Summers, Proc SPIE, 02/2009

Hollow fibre: a biophotonic implant for live cells, Oscar F. Silvestre, Mark D. Holton, Huw D. Summers, Paul J. Smith, Rachel J. Errington, Proc SPIE, 01/2009

Imaging concentric modulations in transverse modes of a vertical-cavity surface emitting laser using a scanning near-field optical microscope, M. D. Holton, P. Rees, P. R. Dunstan, Journal of Applied Physics, 023103-023103-6, 101(2), 01/2007

Fluorescence imaging and investigations of directly labelled chromosomes using scanning near-field optical microscopy, Richard M Baylis, Shareen H Doak, Mark D Holton, Peter R Dunstan, Ultramicroscopy, 308-12, 107(4-5), 01/2007

Improved Schottky contacts to annealed 4H-SiC using a protective carbon cap: Investigated using current voltage measurements and atomic force microscopy, O.J. Guy, D. Doneddu, L. Chen, M.R. Jennings, M.P. Ackland, R. Baylis, M.D. Holton, P. Dunstan, S.P. Wilks, P.A. Mawby, Diamond and Related Materials, 01/2006